Selective transport of long-chain fatty acids by FAT/CD36 in skeletal muscle of broilers

CORC > 厦门大学 > 生命科学－已发表论文

	Selective transport of long-chain fatty acids by FAT/CD36 in skeletal muscle of broilers
	Guo, J. ; Shu, G. ; Zhou, L. ; Zhu, X. ; Liao, W. ; Wang, S. ; Yang, J. ; Zhou, G. ; Xi, Q. ; Gao, P. ; Zhang, Y. ; Zhang, S. ; Yuan, L. ; Jiang, Q. ; Yuan L(袁立)
刊名	http://dx.doi.org/10.1017/S1751731112001619
	2013
关键词	INTRAMUSCULAR FAT BINDING PROTEIN ADIPOSE DIFFERENTIATION 3T3-L1 ADIPOCYTES MEAT OXIDATION CHICKEN HEART MITOCHONDRIA INVOLVEMENT
英文摘要	National Natural Science Foundation of China [u0731004, 30901058, 30972157]; National Basic Research Program of China [2009CB941601]; Natural Science Foundation of Guangdong Province of China [9451064201003790, 9151064201000056]; Specialized Research Fund for the Doctoral Program of Higher Education of China [20094404120012]; Fatty acid translocase (FAT/CD36) is a membrane receptor that facilitates long-chain fatty acid uptake. To investigate its role in the regulation of long-chain fatty acid composition in muscle tissue, we studied and compared FAT/CD36 gene expression in muscle tissues of commercial broiler chickens and Chinese local Silky fowls. The results from gas chromatography-mass spectrometry analysis of muscle samples demonstrated that Chinese local Silky fowls had significantly higher (P < 0.05) proportions of linoleic acid (LA) and palmitic acid, lower proportions (P < 0.05) of arachidonic acid (AA) and oleic acid than the commercial broiler chickens. The mRNA expression levels of fatty acid (FA) transporters (FA transport protein-1, membrane FA-binding protein, FAT/CD36 and caveolin-1) in the m. ipsilateral pectoralis and biceps femoris were analyzed by Q-PCR, and FAT/CD36 expression levels showed significant differences between these types of chickens (P < 0.01). Interestingly, the levels of FAT/CD36 expression are positively correlated with LA content (r = 0.567, P < 0.01) but negatively correlated with palmitic acid content (r = -0.568, P < 0.01). Further experiments in the stably transfected Chinese hamster oocytes cells with chicken FAT/CD36 cDNA demonstrated that overexpression of FAT/CD36 improves total FA uptake with a significant increase in the proportion of LA and AA, and a decreased proportion of palmitic acid. These results suggest that chicken FAT/CD36 may selectively transport LA and AA, which may lead to the higher LA deposition in muscle tissue.
语种	英语
出版者	CAMBRIDGE UNIV PRESS
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90607]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Guo, J.,Shu, G.,Zhou, L.,et al. Selective transport of long-chain fatty acids by FAT/CD36 in skeletal muscle of broilers[J]. http://dx.doi.org/10.1017/S1751731112001619,2013.
APA	Guo, J..,Shu, G..,Zhou, L..,Zhu, X..,Liao, W..,...&袁立.(2013).Selective transport of long-chain fatty acids by FAT/CD36 in skeletal muscle of broilers.http://dx.doi.org/10.1017/S1751731112001619.
MLA	Guo, J.,et al."Selective transport of long-chain fatty acids by FAT/CD36 in skeletal muscle of broilers".http://dx.doi.org/10.1017/S1751731112001619 (2013).