QQ客服 官方微博 反馈留言

	Activation of p38 alpha MAPK enhances collagenase-1 (Matrix metalloproteinase (MMP)-1) and stromelysin-1 (MMP-3) expression by mRNA stabilization
	Reunanen, N. ; Li, S. P. ; Ahonen, M. ; Foschi, M. ; Han, J. H. ; Kahari, V. M. ; Han JH(韩家淮)
	2002
关键词	HUMAN FIBROBLAST COLLAGENASE MESSENGER-RNA STABILITY HUMAN SKIN FIBROBLASTS PROTEIN-KINASE GENE-EXPRESSION I COLLAGEN 3-DIMENSIONAL COLLAGEN GROWTH-FACTOR P38 CELLS
英文摘要	Here, we have examined the role of distinct MAPK pathways in the regulation of collagenase-1 (matrix metalloproteinase (MMP)-1) and stromelysin-1 (MMP-3) expression by human skin fibroblasts. Tumor necrosis factor-alpha rapidly and transiently activated ERK1/2 and JNK in fibroblasts, whereas the activation of p38 MAPK was more persistent. Inhibition of p38 activity by SB203580 markedly (by 80-90%) inhibited induction of MMP-1 and MMP-3 expression by tumor necrosis factor-alpha, whereas blocking the activation of ERK1/2 by PD98059 had no effect. Activation of endogenous ERK1/2 by adenovirus-mediated transfer of constitutively active MEK1 resulted in potent induction of MMP-1 and MMP-3 expression. Activation of endogenous or adenovirally expressed p38alpha by adenovirally delivered constitutively active MKK3b and MKK6b also enhanced MMP-1 and MMP-3 expression and augmented the up-regulatory effect of ERK1/2 activation on the expression of these MMPs. Activation of ERK1/2 resulted in induction of c-jun, junB, and c-fos expression, whereas activation of p38 alone had no effect. In contrast, activation of p38alpha resulted in marked stabilization of MMP-1 and MMP-3 mRNAs. These results identify two distinct and complementary signaling mechanisms mediating induction of MMP-1 and MMP-3 expression in dermal fibroblasts: AP-1-dependent transcriptional activation via the ERK1/2 pathway and AP-1-independent enhancement via p38alpha MAPK by mRNA stabilization. It is conceivable that both modes of action play an important role in controlling the proteolytic phenotype of fibroblasts, e.g. in wound repair and tumor invasion.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65811]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Reunanen, N.,Li, S. P.,Ahonen, M.,et al. Activation of p38 alpha MAPK enhances collagenase-1 (Matrix metalloproteinase (MMP)-1) and stromelysin-1 (MMP-3) expression by mRNA stabilization[J],2002.
APA	Reunanen, N..,Li, S. P..,Ahonen, M..,Foschi, M..,Han, J. H..,...&韩家淮.(2002).Activation of p38 alpha MAPK enhances collagenase-1 (Matrix metalloproteinase (MMP)-1) and stromelysin-1 (MMP-3) expression by mRNA stabilization..
MLA	Reunanen, N.,et al."Activation of p38 alpha MAPK enhances collagenase-1 (Matrix metalloproteinase (MMP)-1) and stromelysin-1 (MMP-3) expression by mRNA stabilization".(2002).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。