Comparison of inactivation and unfolding of green crab (Scylla serrata) alkaline phosphatase during denaturation by guanidinium chloride

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	Comparison of inactivation and unfolding of green crab (Scylla serrata) alkaline phosphatase during denaturation by guanidinium chloride
	Chen, Q. X. ; Zhang, W. ; Zheng, W. Z. ; Zhang, Z. ; Yan, S. X. ; Zhang, T. ; Zhou, H. M. ; Chen QX(陈清西)
刊名	http://dx.doi.org/10.1007/BF01886862
	1996-05
关键词	CONFORMATIONAL-CHANGES CREATINE-KINASE ACTIVE-SITES DEHYDROGENASE HYDROCHLORIDE ENZYME RATES
英文摘要	Green crab (Scylla serrata) alkaline phosphatase (EC 3.1.3.1) is a metalloenzyme, each active site in which contains a tight cluster of two zinc ions and one magnesium ion. Unfolding and inactivation of the enzyme during denaturation in guanidinium chloride (GuHCl) solutions of different concentrations have been compared. The kinetic theory of the substrate reaction during irreversible inhibition of enzyme activity previously described by Tsou [(1988), Adv. Enzymol. Related Areas Mol. Biol. 61, 381-436] has been applied to a study on the kinetics of the course of inactivation of the enzyme during denaturation by GuHCl. The rate constants of unfolding and inactivation have been determined. The results show that inactivation occurs before noticeable conformational change can be detected. It is suggested that the active site of green crab alkaline phosphatase containing multiple metal ions is also situated in a limited region of the enzyme molecule that is more fragile to denaturants than the protein as a whole.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65661]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Chen, Q. X.,Zhang, W.,Zheng, W. Z.,et al. Comparison of inactivation and unfolding of green crab (Scylla serrata) alkaline phosphatase during denaturation by guanidinium chloride[J]. http://dx.doi.org/10.1007/BF01886862,1996.
APA	Chen, Q. X..,Zhang, W..,Zheng, W. Z..,Zhang, Z..,Yan, S. X..,...&陈清西.(1996).Comparison of inactivation and unfolding of green crab (Scylla serrata) alkaline phosphatase during denaturation by guanidinium chloride.http://dx.doi.org/10.1007/BF01886862.
MLA	Chen, Q. X.,et al."Comparison of inactivation and unfolding of green crab (Scylla serrata) alkaline phosphatase during denaturation by guanidinium chloride".http://dx.doi.org/10.1007/BF01886862 (1996).