A rapid and sensitive LC-MS/MS assay for the quantitation of deacetyl mycoepoxydiene in rat plasma with application to preclinical pharmacokinetics studies

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	A rapid and sensitive LC-MS/MS assay for the quantitation of deacetyl mycoepoxydiene in rat plasma with application to preclinical pharmacokinetics studies
	Gao, Yanhui ; Xu, Jiangbo ; Xu, Jiaxing ; Huang, Yaojian ; Shen, Yuemao ; Liu, ZhaoPing ; Shen YM(沈月毛)
刊名	http://dx.doi.org/10.1016/j.jchromb.2011.06.044
	2012-01-01
关键词	DEACETYLMYCOEPOXYDIENE CHROMATOGRAPHY METABOLITES
英文摘要	National High Technology Research and Development Program (863) of China [2007AA091503]; The purpose of this study was to develop and validate a high-performance liquid chromatographic-tandem mass spectrometric (LC-MS/MS) method for analysis of the deacetyl mycoepoxydiene in rat plasma. The analyte and internal standard (IS.), benorilate, were extracted from rat plasma by precipitation protein and separated on a C(18) column using acetonitrile-0.5% formic acid as mobile phase. Detection was performed using a turbo-spray ionization source and mass spectrometric positive multi-reaction-monitoring-mode (+MRM) at an ion voltage of +4800 V. The assay was linear over the concentration range 5-5000 ng/mL with the lowest limit of quantification (LLOQ) of 5 ng/mL. The method also afforded satisfactory results in terms of the sensitivity, specificity, precision (Ultra- and inter-day, RSD <5.8%), accuracy, recovery as well as the stability of the analyte under various conditions. The method was successfully applied to a preclinical pharmacokinetic study in rats after a single intravenous administration of deacetyl mycoepoxydiene 10 mg/kg. (C) 2011 Published by Elsevier B.V.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65365]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Gao, Yanhui,Xu, Jiangbo,Xu, Jiaxing,et al. A rapid and sensitive LC-MS/MS assay for the quantitation of deacetyl mycoepoxydiene in rat plasma with application to preclinical pharmacokinetics studies[J]. http://dx.doi.org/10.1016/j.jchromb.2011.06.044,2012.
APA	Gao, Yanhui.,Xu, Jiangbo.,Xu, Jiaxing.,Huang, Yaojian.,Shen, Yuemao.,...&沈月毛.(2012).A rapid and sensitive LC-MS/MS assay for the quantitation of deacetyl mycoepoxydiene in rat plasma with application to preclinical pharmacokinetics studies.http://dx.doi.org/10.1016/j.jchromb.2011.06.044.
MLA	Gao, Yanhui,et al."A rapid and sensitive LC-MS/MS assay for the quantitation of deacetyl mycoepoxydiene in rat plasma with application to preclinical pharmacokinetics studies".http://dx.doi.org/10.1016/j.jchromb.2011.06.044 (2012).