Gut SCP is an immune-relevant molecule involved in the primary immunological memory or pattern recognition in the amphioxus Branchiostoma belcheri

CORC > 厦门大学 > 生命科学－已发表论文

	Gut SCP is an immune-relevant molecule involved in the primary immunological memory or pattern recognition in the amphioxus Branchiostoma belcheri
	Zhuang, Z. H. ; Li, H. ; Yang, J. N. ; Liu, X. A. ; Gao, Y. Y. ; Li, Q. F. ; Wang, S. Y. ; Peng, X. X. ; Li QF(李祺福)
刊名	http://dx.doi.org/10.1016/j.fsi.2010.12.016
	2011-02
关键词	CALCIUM-BINDING PROTEINS INVERTEBRATE IMMUNITY IDENTIFICATION ANGUILLARUM EXPRESSION PROTECTION AEROMONAS REVEALS DISEASE KINASE
英文摘要	National Basic Research Program of China [2006CB101807]; NSFC [30530610]; Guangdong NSF [7117645]; To understand the role of calcium-binding proteins of invertebrates in immunological response, amphioxus sarcoplasmic calcium-binding protein (SCP) was investigated in the present study. Following gene cloning, recombinant protein expression and purification and antibody preparation, the expression and alteration of SCP in the response to bacterial challenge were detected using Western blotting. SCP was not detected in the branchia, humoral fluid, gonad or in the gut of wounded animals, but it was abundant in muscle and appeared in the gut of healthy animals using Vibrio parahaemolyticus immunization and challenge. Furthermore, whether gut SCP possessed anamnestic response was investigated using cross-immune challenge between Gram-positive and negative bacteria. Gut SCP showed stronger anamnestic activity or pattern-recognition in response to Gram-negative bacterium V parahaemolyticus than Gram-positive bacterium Staphylococcus aureus. The response was faster and more species-specific to V parahaemolyticus, whereas it was slower and longer to S. aureus. The reason why the response showed significant difference between Gram-positive and -negative bacteria awaits investigation. These results indicate that gut SCP is an immune-relevant molecule involved in the primary immunological memory or pattern recognition in the amphioxus Branchiostoma belcheri. (C) 2010 Elsevier Ltd. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65172]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Zhuang, Z. H.,Li, H.,Yang, J. N.,et al. Gut SCP is an immune-relevant molecule involved in the primary immunological memory or pattern recognition in the amphioxus Branchiostoma belcheri[J]. http://dx.doi.org/10.1016/j.fsi.2010.12.016,2011.
APA	Zhuang, Z. H..,Li, H..,Yang, J. N..,Liu, X. A..,Gao, Y. Y..,...&李祺福.(2011).Gut SCP is an immune-relevant molecule involved in the primary immunological memory or pattern recognition in the amphioxus Branchiostoma belcheri.http://dx.doi.org/10.1016/j.fsi.2010.12.016.
MLA	Zhuang, Z. H.,et al."Gut SCP is an immune-relevant molecule involved in the primary immunological memory or pattern recognition in the amphioxus Branchiostoma belcheri".http://dx.doi.org/10.1016/j.fsi.2010.12.016 (2011).