QQ客服 官方微博 反馈留言

	Identification of up-regulated genes in amphioxus neurula and the expression of AmphiFABP
	Liu, Xiaohui ; Sun, Yi ; Zhang, Juyong ; Li, Guang ; Li G(李光) ; Wang, Yiquan ; Wang YQ(王义权)
	2010-11-20
关键词	amphioxus AmphiFABP embryonic development subtracted cDNA library suppression subtractive hybridization (SSH)
英文摘要	Amphioxus is a good model organism for understanding the origin and developmental mechanism of vertebrates owing to its important evolutionary position. During the developmental process of amphioxus embryo, the neurula is a crucial stage because of neural tube and notochord formation as well as somite emergence at this stage. In order to isolate genes up-regulated at the neurula stage, we constructed an 11-hour neurula subtracted cDNA library of amphioxus Branchiostoma belcheri and sequenced 204 ESTs representing 82 contigs. Comparative analysis revealed that 55% of those contigs were homologous to various known genes while 45% of them had no significant similarity to any known genes. Those observations imply that the un-identified ESTs might contain some new genes which are involved in the development of amphioxus neurula. Real-time quantitative PCR (RTqPCR) indicated that the expression levels of 14 genes are up-regulated after gastrulation among 20 assayed genes. Of those up-regulated genes, we further cloned and sequenced the full-length of fatty acid binding protein gene (AmphiFABP). The deduced protein sequence was similar to that of vertebrate brain FABP and heart FABP, and in situ hybridization displayed that AmphiFABP, similar to their vertebrate cognates, was expressed not only in nervous system but also in embryonic somite and gut, hinting a multifunctional property of AmphiFABP in amphioxus.; National Natural Science Foundation of China[30830023]; National High-tech Research and Development Program ("863"Program) of China[2008AA092602]
语种	英语
出版者	GENETICS SOC JAPAN
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1266/ggs.86.37]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Liu, Xiaohui,Sun, Yi,Zhang, Juyong,et al. Identification of up-regulated genes in amphioxus neurula and the expression of AmphiFABP[J],2010.
APA	Liu, Xiaohui.,Sun, Yi.,Zhang, Juyong.,Li, Guang.,李光.,...&王义权.(2010).Identification of up-regulated genes in amphioxus neurula and the expression of AmphiFABP..
MLA	Liu, Xiaohui,et al."Identification of up-regulated genes in amphioxus neurula and the expression of AmphiFABP".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。