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	Construction of a ferritin reactor: An efficient means for trapping various heavy metal ions in flowing seawater
	Huang, HQ ; Huang HQ(黄河清) ; Lin, QM ; Lou, ZB
	2000
关键词	ferritin reactor heavy metal ions trapping and storage seawater monitoring pollution
英文摘要	An apparatus consisting of two pumps, a mixer, a ferritin reactor, and a spectrophotometer was constructed to study the ability to trap various heavy metal ions (M2+) and the dynamics of a reconstituted ferritin reactor in flowing seawater. Reconstituted pig spleen ferritin (PSFr) is assembled from ape-protein shell to form a reconstituted iron core. The main components of the PSF, are its core, which contains an Fe2+:P-i stoichiometry of 6.0+/-0.5, reconstituted from pig spleen apoferritin (apo PSF), Fe2+, inorganic phosphate (P-i), and O-2 (0.6 atm). The Fe3+-P-i clusters within the PSFr core exhibit resistance to salt ranging from 1% to 6% NaCl. The ferritin reactor consists of PSFr and an oscillating bag. Using the reactor, M2+ ions such as Cd2+, Zn2+, Co2+, and Mn2+ are directly trapped by the ferritin. We found a 1:2+/-0.2 stoichiometry of the trapped M2+ to the released iron as measured by chemical analysis or atomic absorption spectrometry; nontransient elements such as Na+, K+, Ca2+, etc., were scarcely trapped by the reactor. This study provides basic conditions for establishing a ferritin reactor and a convenient means for monitoring the pollution of heavy metal ions in seawater.
语种	英语
出版者	KLUWER ACADEMIC/PLENUM PUBL
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1023/A:1026541129563]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Huang, HQ,Huang HQ,Lin, QM,et al. Construction of a ferritin reactor: An efficient means for trapping various heavy metal ions in flowing seawater[J],2000.
APA	Huang, HQ,黄河清,Lin, QM,&Lou, ZB.(2000).Construction of a ferritin reactor: An efficient means for trapping various heavy metal ions in flowing seawater..
MLA	Huang, HQ,et al."Construction of a ferritin reactor: An efficient means for trapping various heavy metal ions in flowing seawater".(2000).

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