QQ客服 官方微博 反馈留言

	Importin 13 Serves as a Potential Marker for Corneal Epithelial Progenitor Cells
	Wang, Hua ; Mao, Yi-Hui ; Li, Wei ; Peng, Juan ; Tan, Gang ; Zhou, Yue-Ping ; Zhong, Jing-Xiang ; Liu, Zu-Guo ; Tao, Tao ; Tao T(陶涛) ; Tseng, Scheffer C. G.（TissueTech & Ocular Surface Ctr, Miami, FL USA） ; Kawakita, Tetsuya（ Keio Univ, Sch Med, Dept Ophthalmol, Tokyo, Japan）
	2009
关键词	Importin13 Cornea Epithelium Progenitor cell Limbal stem cell
英文摘要	Importin13 (IPO13), the newest member of importin-beta family discovered recently, is a unique nucleus-cytoplasm bidirectional transport receptor protein. In this study, IPO13 expression in human corneal tissue, limbal epithelial primary explant and clonal culture was evaluated by immunostaining and reverse-transcription polymerase chain reasgon. IPO13 function was evaluated in the corneal epithelial culture treated with IPO13 inhibitor, or fetal bovine serum (FBS)-containing Dulbecco's modified Eagle's medium (DMEM) medium by colony-forming efficiency, clone growth capacity, MTT, immunostaining, and Western blotting assay. IPO13 protein was expressed mainly in nuclei of limbal epithelial basal cells, but not in the other cell layers of limbus and full thickness of corneal epithelia. IPO13 was expressed in the majority of epithelial cells in early-stage clones and in the margin of late-stage clones. IPO13 was positively expressed in mouse TKE2 progenitor cells cultured in keratinocyte serum-free defined medium, while it became negative in FBS-containing DMEM, which promoted TKE2 cell differentiation. In the presence of IPO13 inhibitor, IPO13 expression and the proliferative capacity decreased in human limbal epithelial clones and mouse TKE2 cells, which were accompanied with the cell differentiation. In conclusion, our findings demonstrate for the first time that IPO13 is uniquely expressed by human limbal basal epithelial cells, and plays an important role in maintaining the phenotype, high proliferative potential, and less differentiation of corneal epithelial progenitor cells, suggesting that IPO13 could serve as a novel potential marker for corneal epithelial progenitor cells. STEM CELLS 2009;27:2516-2526; National Natural Science Fund of China [30700926]; National High Technology Research and Development Program of China [2006AA02A131]; Postdoctorate Fellowship of China [20070410807]; Natural Science Fund of Fujian Province [2008J0113]; Medical Innovation Project of Fujian Province [2007-CX-19]; Science and Technology Project of Xiamen [3502Z20074032]
语种	英语
出版者	ALPHAMED PRESS
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1002/stem.174]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Wang, Hua,Mao, Yi-Hui,Li, Wei,et al. Importin 13 Serves as a Potential Marker for Corneal Epithelial Progenitor Cells[J],2009.
APA	Wang, Hua.,Mao, Yi-Hui.,Li, Wei.,Peng, Juan.,Tan, Gang.,...&Kawakita, Tetsuya.(2009).Importin 13 Serves as a Potential Marker for Corneal Epithelial Progenitor Cells..
MLA	Wang, Hua,et al."Importin 13 Serves as a Potential Marker for Corneal Epithelial Progenitor Cells".(2009).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。