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	Asymmetric synthesis of ethyl (R)-4-chloro-3-hydroxybutanoate with a two recombinant Escherichia coli system
	Jing, Ke-Ju ; Ban, Wen-Ting ; Jing KJ(敬科举)
	2013
关键词	Bacteriology Organic solvents
英文摘要	Conference Name:4th International Conference on Manufacturing Science and Engineering, ICMSE 2013. Conference Address: Dalian, China. Time:March 30, 2013 - March 31, 2013.; Northeastern University, China; Harbin Institute of Technology; Jilin University; The asymmetric reduction of ethyl 4-chloro-acetoacetate (CAAE) to ethyl (R)-4-chloro-3- hydroxybutanoate (R-CHBE) biocatalysed by the aldehyde reductase of Sporobolomyces salmonicolor expressed in E. coli M15 (pQE30-alr) in combination with regeneration of NADPH by the glucose dehydrogenase of Bacillus megaterium expressed in E. coli M15 (pQE30-gdh) was reported. The bioreduction was carried out in a two-phase reaction system with n-butyl acetate as an organic solvent. Bioconversion of 300 mmol CAAE with a final yield of 97.5% and an enantiometric excess of 99% was achieved without the addition of cofactor NADPH. ? (2013) Trans Tech Publications, Switzerland.
语种	英语
出处	http://dx.doi.org/10.4028/www.scientific.net/AMR.690-693.1188
出版者	TRANS TECH PUBLICATIONS LTD
内容类型	其他
源URL	[http://dspace.xmu.edu.cn/handle/2288/85411]
专题	化学化工－会议论文
推荐引用方式 GB/T 7714	Jing, Ke-Ju,Ban, Wen-Ting,Jing KJ. Asymmetric synthesis of ethyl (R)-4-chloro-3-hydroxybutanoate with a two recombinant Escherichia coli system. 2013-01-01.

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