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	超高效液相色谱法检测角鲨烯; Determination of Squalene by Ultra Performance Liquid Chromatography
	陈伟珠 ; 方华 ; 张怡评 ; 洪专
	2014-12-25
关键词	角鲨烯 超高效液相色谱 测定 squalene ultra performance liquid chromatography(UPLC) determination
英文摘要	应用超高效液相色谱法,建立了角鲨烯的测定方法。采用uPlC bEH C18(2.1MMx50MM,1.7μM)色谱柱考察了流动相有机相比例、流速及柱温对角鲨烯样品分离的影响,确定了最佳色谱条件:等度洗脱,流动相为甲醇-水(95∶5,体积比),流速0.4M l/MIn,柱温35℃,检测波长210nM。该方法下,角鲨烯在0.2-10μg/M l范围内浓度和面积呈现良好的线性关系,相关系数r为0.9998;检测限为0.1ng,定量限分别为0.25ng,精密度试验rSd(n=6)为0.36%,平均回收率为100.82%(rSd=0.99%)。本方法快速、简单、可靠、灵敏、重复性好,可用于角鲨烯有关样品的快速检测。; The article is researching determination of squalene by Ultra performance liquid chromatography(UPLC).The analysis was performed on an Acquity UPLC BEH C18 column(2.1mm×50mm,1.7μm).The influence of flow rate,the proportion of organic phase in mobile phase,and column temperature on the separation of squalene was comprehensively studied.The optimal separation condition was as follows:a mobile phase consisting of methanol and water(95∶5,v/v) with a isocratic elution profile,UVdetection wavelength at 210 nm,0.4 m L/min of flow rate for mobile phase,35℃ of column temperature.On the above condition,good linear was observed in the linear range of 0.2-10 μg/m L,and the correlation coefficient r was 0.9998.The limits of detection(S/N=3) were 0.1ng and the limits of quantification(S/N=10) were 0.25 ng.The average recovery rates was 100.82%(RSD=0.99%).The RSD of repetition was 0.36%(n=6).This method was simple,accurate and sensitive with a good reproducibility.It is suitable for fast detection of squalene.; 厦门海洋研究开发院共建项目(2014); 海洋生物技术产业化中试技术研发公共服务平台(12PZP001SF10); 广东海洋经济发展区域示范项目(GD2012-D01-001)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/106941]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	陈伟珠,方华,张怡评,等. 超高效液相色谱法检测角鲨烯, Determination of Squalene by Ultra Performance Liquid Chromatography[J],2014.
APA	陈伟珠,方华,张怡评,&洪专.(2014).超高效液相色谱法检测角鲨烯..
MLA	陈伟珠,et al."超高效液相色谱法检测角鲨烯".(2014).

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