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	基于氧化还原酶活性时变研究巴利阿里假单胞菌中心碳代谢; Analysis of Central Carbon Metabolism in Pseudomonas balearica Based on Key Oxidoreductases Detecting
	温卫卫 ; 周小芬 ; 王世珍 ; 方柏山
	2013-01-28
关键词	海洋微生物 中心碳代谢 氧化还原酶 动力学 marine bacteria central carbon metabolism oxidoreductase kinetics
英文摘要	以分离自中国北部湾沉积物的海洋菌株巴利阿里假单胞菌(PSEudOMOnAS bAlEArICA)为例,在考察其生长动力学的同时,监测了其中心碳代谢(CCM)中6种关键氧化还原酶,葡萄糖-6-磷酸脱氢酶、丙酮酸脱氢酶、2-酮戊二酸脱氢酶、苹果酸脱氢酶、苹果酸酶及异柠檬酸脱氢酶的酶活性时变,从酶蛋白表达水平揭示了海洋生长条件下巴利阿里假单胞菌CCM中关键氧化还原酶的酶活性变化的规律,为研究微生物CCM和发掘海洋微生物新型酶制剂提供一种新思路.; Assaying the central carbon metabolism(CCM) through the enzyme′s level may be one effective method to reveal the interaction between the adaptability of marine bacteria and the extreme environmental conditions.Marine bacteria may have different CCM with terrestrial bacteria as they have different growth environment.So it is valuable to research the related key oxidoreductases,which is important to uncover the CCM of marine bacteria.The response of central carbon metabolism of marine bacteria to incubation time was investigated based on key oxidoreductases detecting,while traditional metabolic analysis was carried out by 13C metabolic flux analysis.For example,Pseudomonas balearica isolated from sediment of China's offshore was investigated.Besides growth kinetics,the central carbon metabolism of P.balearica was uncovered by a comprehensive analysis of six key oxidoreductases,glucose-6-phosphate dehydrogenase,pyruvate dehydrogenase,2-ketoglutarate dehydrogenase,malate dehydrogenase,malic enzyme and isocitrate dehydrogenase.The changing regularity of key oxidoreductases activities of central carbon metabolism in P.balearica was revealed on protein level.A new technique for the study of microbial central carbon metabolism and the excavation of new enzyme preparations from marine microbial was provided.; 国家自然科学基金项目(41176111); 福建省高校产学合作科技重大项目(2010H6023); 福建省自然科学基金项目(2012J01049)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/106826]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	温卫卫,周小芬,王世珍,等. 基于氧化还原酶活性时变研究巴利阿里假单胞菌中心碳代谢, Analysis of Central Carbon Metabolism in Pseudomonas balearica Based on Key Oxidoreductases Detecting[J],2013.
APA	温卫卫,周小芬,王世珍,&方柏山.(2013).基于氧化还原酶活性时变研究巴利阿里假单胞菌中心碳代谢..
MLA	温卫卫,et al."基于氧化还原酶活性时变研究巴利阿里假单胞菌中心碳代谢".(2013).

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