Kinetic and Sensitive Analysis of Tyrosinase Activity Using Electron Transfer Complexes: In Vitro and Intracellular Study

CORC > 厦门大学 > 化学化工－已发表论文

	Kinetic and Sensitive Analysis of Tyrosinase Activity Using Electron Transfer Complexes: In Vitro and Intracellular Study
	Zhu, Xianglong ; Hu, Juan ; Zhao, Zhenghuan ; Sun, Mingjun ; Chi, Xiaoqin ; Wang, Xiaomin ; Gao, Jinhao ; Chi XQ(池小琴) ; Wang XM(王效民) ; Gao JH(高锦豪)
刊名	http://dx.doi.org/10.1002/smll.201401595
	2014
关键词	Amino acids Dermatology Diagnosis Electrons Enzyme activity Enzymes Neurodegenerative diseases Oncology Quenching Scaffolds (biology)
英文摘要	Tyrosinase is an important marker of human diseases such as the neurodegeneration associated with Parkinson's disease and melanoma. Sensitive detection of tyrosinase activity in vitro and inside cells is of great significance to medical diagnostics and skin disorder treatments. With unique photophysical properties, semiconductor quantum dots (QDs) are employed as photoluminescent platforms for various biosensing, in particular for the detection of enzyme activities. In this work, QDs are functionalized with tyrosine and zwitterionic molecules to construct a nanometer-scale scaffold (QD-Tyr conjugate), and this is used to test tyrosinase activity in vitro and inside cells. Tyrosinase oxidizes tyrosine to dopachrome and switches on the electron-transfer access, which relates to fluorescence quenching. High quenching efficiency is achieved by shortening the distance between the electron donors and acceptors, which is attributed to the small size of the conjugated tyrosine. Enzymatic process curves reveal the enhanced enzymatic activity on the conjugated nanoparticle substrate, which leads to highly sensitive detection of tyrosinase (as low as 1 nM). It is also demonstrated that QD-Tyr conjugates can sensitively probe intracellular tyrosinase in melanoma cells, which promises great potential in disease monitoring and medical diagnostics.
语种	英语
出版者	Wiley-VCH Verlag
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89776]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Zhu, Xianglong,Hu, Juan,Zhao, Zhenghuan,et al. Kinetic and Sensitive Analysis of Tyrosinase Activity Using Electron Transfer Complexes: In Vitro and Intracellular Study[J]. http://dx.doi.org/10.1002/smll.201401595,2014.
APA	Zhu, Xianglong.,Hu, Juan.,Zhao, Zhenghuan.,Sun, Mingjun.,Chi, Xiaoqin.,...&高锦豪.(2014).Kinetic and Sensitive Analysis of Tyrosinase Activity Using Electron Transfer Complexes: In Vitro and Intracellular Study.http://dx.doi.org/10.1002/smll.201401595.
MLA	Zhu, Xianglong,et al."Kinetic and Sensitive Analysis of Tyrosinase Activity Using Electron Transfer Complexes: In Vitro and Intracellular Study".http://dx.doi.org/10.1002/smll.201401595 (2014).