Europium-labeled activity-based probe through click chemistry: Absolute serine protease quantification using 153Eu isotope dilution ICP/MS | |
Yan, Xiaowen ; Luo, Yacui ; Zhang, Zhubao ; Li, Zhaoxin ; Luo, Qiang ; Yang, Limin ; Zhang, Bo ; Chen, Haifeng ; Bai, Peiming ; Wang, Qiuqua ; Yang LM(杨利民) ; Zhang B(张博) ; Chen HF(陈海峰) ; Bai PM(白培明) ; Wang QQ(王秋泉) | |
刊名 | http://dx.doi.org/10.1002/anie.201108277 |
2012 | |
关键词 | Amino acids Isotopes Liquid chromatography Probes Proteins Synthesis (chemical) |
英文摘要 | Click and analyze: The titled probe was synthesized by conjugating a sulfonyl fluoride and azido unit using click chemistry to give SF-Eu, which can react specifically with serine (Ser) in the active site of serine protease (SP). Combination of the method with 153Eu-isotope dilution ICP/MS enables absolute protein quantification of active SPs in biological samples using only one 153Eu(NO3)3 isotopic standard. Copyright 漏 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
语种 | 英语 |
出版者 | Wiley-VCH Verlag |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/89548] |
专题 | 化学化工-已发表论文 |
推荐引用方式 GB/T 7714 | Yan, Xiaowen,Luo, Yacui,Zhang, Zhubao,et al. Europium-labeled activity-based probe through click chemistry: Absolute serine protease quantification using 153Eu isotope dilution ICP/MS[J]. http://dx.doi.org/10.1002/anie.201108277,2012. |
APA | Yan, Xiaowen.,Luo, Yacui.,Zhang, Zhubao.,Li, Zhaoxin.,Luo, Qiang.,...&王秋泉.(2012).Europium-labeled activity-based probe through click chemistry: Absolute serine protease quantification using 153Eu isotope dilution ICP/MS.http://dx.doi.org/10.1002/anie.201108277. |
MLA | Yan, Xiaowen,et al."Europium-labeled activity-based probe through click chemistry: Absolute serine protease quantification using 153Eu isotope dilution ICP/MS".http://dx.doi.org/10.1002/anie.201108277 (2012). |
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