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	Relationship of trehalose accumulation with ethanol fermentation in industrial Saccharomyces cerevisiae yeast strains
	Wang, Pin-Mei ; Zheng, Dao-Qiong ; Chi, Xiao-Qin ; Li, Ou ; Qian, Chao-Dong ; Liu, Tian-Zhe ; Zhang, Xiao-Yang ; Du, Feng-Guang ; Sun, Pei-Yong ; Qu, Ai-Min ; Wu, Xue-Chang ; Chi XQ(池小琴)
刊名	http://dx.doi.org/10.1016/j.biortech.2013.11.033
	2014
关键词	ACID TREHALASE CELL VIABILITY SALINE STRESS TOLERANCE IMPROVE OVEREXPRESSION MOBILIZATION COMBINATION RESISTANCE PROMOTES
英文摘要	Scientific Research Fund of Zhejiang Provincial Education Department [Y201327852]; State Key Laboratory of Motor Vehicle Biofuels Technology of China [2013013]; Postdoctoral Science Foundation of China [2013M531450]; Postdoctoral Science Foundation of Zhejiang province [BSH1301026]; The protective effect and the mechanisms of trehalose accumulation in industrial Saccharomyces cerevisiae strains were investigated during ethanol fermentation. The engineered strains with more intercellular trehalose achieved significantly higher fermentation rates and ethanol yields than their wild strain ZS during very high gravity (VHG) fermentation, while their performances were not different during regular fermentation. The VHG fermentation performances of these strains were consistent with their growth capacity under osmotic stress and ethanol stress, the key stress factors during VHG fermentation. These results suggest that trehalose accumulation is more important for VHG fermentation of industrial yeast strains than regular one. The differences in membrane integrity and antioxidative capacity of these strains indicated the possible mechanisms of trehalose as a protectant under VHG condition. Therefore, trehalose metabolic engineering may be a useful strategy for improving the VHG fermentation performance of industrial yeast strains. (C) 2013 Elsevier Ltd. All rights reserved.
语种	英语
出版者	ELSEVIER SCI LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89477]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Wang, Pin-Mei,Zheng, Dao-Qiong,Chi, Xiao-Qin,et al. Relationship of trehalose accumulation with ethanol fermentation in industrial Saccharomyces cerevisiae yeast strains[J]. http://dx.doi.org/10.1016/j.biortech.2013.11.033,2014.
APA	Wang, Pin-Mei.,Zheng, Dao-Qiong.,Chi, Xiao-Qin.,Li, Ou.,Qian, Chao-Dong.,...&池小琴.(2014).Relationship of trehalose accumulation with ethanol fermentation in industrial Saccharomyces cerevisiae yeast strains.http://dx.doi.org/10.1016/j.biortech.2013.11.033.
MLA	Wang, Pin-Mei,et al."Relationship of trehalose accumulation with ethanol fermentation in industrial Saccharomyces cerevisiae yeast strains".http://dx.doi.org/10.1016/j.biortech.2013.11.033 (2014).

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