Electrospray ionization mass spectrometric studies on the characteristic fragmentation of Asp/cyclen conjugates

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	Electrospray ionization mass spectrometric studies on the characteristic fragmentation of Asp/cyclen conjugates
	Ma, Chunying ; Li, Chao ; Luan, Xingrong ; Zhang, Jin ; Qiao, Renzhong ; Zhao, Yufen ; Zhao YF(赵玉芬)
刊名	http://dx.doi.org/10.1002/rcm.6822
	2014-03-30
关键词	PEPTIDE IONS ADJACENT GAS-PHASE CLEAVAGE PROTONATED PEPTIDES ACID-RESIDUES ASPARTIC-ACID CHEMISTRY PATHWAYS BOND
英文摘要	National Nature Science Foundation of China [21232005, 21372024, 21202005, 21172016]; RATIONALEDifferentiation and structural characterization of Asp/cyclen conjugates by electrospray ionization tandem mass spectrometry (ESI-MSn) are significantly important for their biomedical application. Hence, the present study is conducted. METHODSThe fragmentations of Asp/cyclen conjugates generated by positive ion mode electrospray ionization were examined here by low-energy collision-induced dissociation (CID). ESI-MSn spectra of cyclen were acquired to confirm cyclen contraction products derived from the studied compounds. The fragments derived from the Asp/cyclen conjugates were proved by deuterium-exchange experiments. RESULTSAsp/cyclen conjugates displayed characteristic dissociation pathways, including cleavages of amide bonds, loss of NH3 and cyclen contraction pathways. It was observed that cleavages of C-terminal amide bonds generated b(2) and b(2)+H2O ions from the protonated CyclenAspAspAsp and a b(1)+H2O ion from the protonated CyclenAspAsp. In addition, various cyclen contraction products were also observed. CONCLUSIONSIn ESI-MSn spectra of studied compounds, fragments of b(n-1)+H2O or cyclic anhydride were generated due to facile mobilization of C-terminal or side-chain COOH protons. In addition, the cyclen contraction products were detected. These results might provide sufficient information for the identification of Asp/cyclen conjugates by mass spectrometry. Copyright (c) 2014 John Wiley & Sons, Ltd.
语种	英语
出版者	WILEY-BLACKWELL
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/89344]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Ma, Chunying,Li, Chao,Luan, Xingrong,et al. Electrospray ionization mass spectrometric studies on the characteristic fragmentation of Asp/cyclen conjugates[J]. http://dx.doi.org/10.1002/rcm.6822,2014.
APA	Ma, Chunying.,Li, Chao.,Luan, Xingrong.,Zhang, Jin.,Qiao, Renzhong.,...&赵玉芬.(2014).Electrospray ionization mass spectrometric studies on the characteristic fragmentation of Asp/cyclen conjugates.http://dx.doi.org/10.1002/rcm.6822.
MLA	Ma, Chunying,et al."Electrospray ionization mass spectrometric studies on the characteristic fragmentation of Asp/cyclen conjugates".http://dx.doi.org/10.1002/rcm.6822 (2014).