A fluorescent double-network-structured hybrid nanogel as embeddable nanoglucometer for intracellular glucometry

CORC > 厦门大学 > 化学化工－已发表论文

	A fluorescent double-network-structured hybrid nanogel as embeddable nanoglucometer for intracellular glucometry
	Fan, Jiao ; Jiang, Xiaomei ; Hu, Yumei ; Si, Yan ; Ding, Li ; Wu, Weitai ; Wu WT(吴伟泰)
刊名	http://dx.doi.org/10.1039/c2bm00162d
	2013
关键词	VOLUME PHASE-TRANSITION PHYSIOLOGICAL PH QUANTUM DOTS OPTICAL SENSORS GLUCOSE-HOMEOSTASIS MASS-SPECTROMETRY METABOLITE LEVELS LIGHT-SCATTERING HIGH-SENSITIVITY DYNAMIC CHANGES
英文摘要	National Natural Science Foundation of China [21274118, 91227120]; Program for New Century Excellent Talents in Fujian Province University; Fundamental Research Funds for the Central Universities [2012121016]; National Found for Fostering Talents of Basic Science [J1030415]; The development of embeddable and remotely interrogatable nanomaterials that allow dynamic quantification of intracellular glucose levels can contribute to a better understanding of physiology. We develop a fluorescent hybrid nanogel glucometer (FNG) that is applicable for intracellular glucometry. Such a FNG (<200 nm) is comprised of ZnO quantum dots covalently bonded onto a loosely-crosslinked gel network of poly(acrylamide), which is interpenetrated in another relatively highly-crosslinked gel network of poly(N-isopropylacrylamide-co-2-acrylamidomethyl-5-fluorophenylboronic acid). This newly developed double-network-structured FNG can adapt to surrounding media of varying glucose levels, and convert the disruptions in homeostasis of glucose level with high reversibility, sensitivity, and selectivity into fluorescence signals at a fast time response. We demonstrate that the FNG can enter the model B16F10 cells and employ the signal transduction ability for fluorescent intracellular glucometry. Furthermore, we show that intracellular glucose level variations associated with a model biological reaction process can be monitored with a high glucose resolution by using the FNG embedded in cells, whilst the reaction mechanism remains nearly unchanged.
语种	英语
出版者	ROYAL SOC CHEMISTRY
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88679]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Fan, Jiao,Jiang, Xiaomei,Hu, Yumei,et al. A fluorescent double-network-structured hybrid nanogel as embeddable nanoglucometer for intracellular glucometry[J]. http://dx.doi.org/10.1039/c2bm00162d,2013.
APA	Fan, Jiao.,Jiang, Xiaomei.,Hu, Yumei.,Si, Yan.,Ding, Li.,...&吴伟泰.(2013).A fluorescent double-network-structured hybrid nanogel as embeddable nanoglucometer for intracellular glucometry.http://dx.doi.org/10.1039/c2bm00162d.
MLA	Fan, Jiao,et al."A fluorescent double-network-structured hybrid nanogel as embeddable nanoglucometer for intracellular glucometry".http://dx.doi.org/10.1039/c2bm00162d (2013).