Transcriptional profile of shrimp white spot syndrome virus (WSSV) genes with DNA microarray

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	Transcriptional profile of shrimp white spot syndrome virus (WSSV) genes with DNA microarray
	Lan, Y. ; Xu, X. ; Yang, F. ; Zhang, X. ; Xu X(徐昕) ; Zhang X(张弦)
刊名	http://dx.doi.org/10.1007/s00705-006-0751-x
	2006-09
关键词	POLYMERASE-CHAIN-REACTION IN-SITU HYBRIDIZATION BACULOVIRUS WSBV GENOME SEQUENCE IDENTIFICATION PRAWN
英文摘要	In an attempt to obtain the transcriptional profile of shrimp white spot syndrome virus (WSSV) genes, DNA microarray analysis was performed using amplified DNA fragments of the WSSV genome that covered most of the presumptive open reading frames (ORFs). Total RNAs were extracted from WSSV-infected crayfish (Cambarus clarkii) and reverse transcribed into cDNA and labeled with P-32-dATP. The DNA microarray detected 81.1% of the ORFs, and 47 of these were transcribed at 6 h post infection, suggesting they may be early genes. Some early genes were further confirmed by temporal analyses of gene transcription. Results in this study indicated the effectiveness of DNA microarray for discovering early viral genes.
语种	英语
出版者	ARCH VIROL
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88546]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Lan, Y.,Xu, X.,Yang, F.,et al. Transcriptional profile of shrimp white spot syndrome virus (WSSV) genes with DNA microarray[J]. http://dx.doi.org/10.1007/s00705-006-0751-x,2006.
APA	Lan, Y.,Xu, X.,Yang, F.,Zhang, X.,徐昕,&张弦.(2006).Transcriptional profile of shrimp white spot syndrome virus (WSSV) genes with DNA microarray.http://dx.doi.org/10.1007/s00705-006-0751-x.
MLA	Lan, Y.,et al."Transcriptional profile of shrimp white spot syndrome virus (WSSV) genes with DNA microarray".http://dx.doi.org/10.1007/s00705-006-0751-x (2006).