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	Surface chargemodulatedaptasensorinasingleglassconical nanopore
	Sheng-Lin Cai ; Shuo-HuiCao ; Yu-BinZheng ; ShuangZhao ; Jin-LeiYang ; Yao-QunLi ; Li YQ(李耀群)
	2015-09-15
关键词	Single glass conical nanopores Surface charge neutralization Ionic current Aptamer Protein sensing
英文摘要	通讯作者地址: Li, YQ; In this work, we have proposed a label-free nanopore-based biosensing strategy for protein detection by performing the DNA-protein interaction inside a single glass conical nanopore. A lysozyme binding aptamer (LBA) was used to functionalize the walls of glass nanopore via siloxane chemistry and negatively charged recognition sites were thus generated. The covalent modification procedures and their recognition towards lysozyme of the single conical nanopore were characterized via ionic current passing through the nanopore membrane, which was measured by recording the current-voltage (I-V) curves in 1 mM KCl electrolyte at pH=7.4. With the occurring of recognition event, the negatively charged wall was partially neutralized by the positively charged lysozyme molecules, leading to a sensitive change of the surface charge-dependent current-voltage (I-V) characteristics. Our results not only demonstrate excellent selectivity and sensitivity towards the target protein, but also suggest a route to extend this nanopore-based sensing strategy to the biosensing platform designs of a wide range of proteins based on a charge modulation.; National Natural Science Foundation of China 21375111 21127005 20975084 973 Program of China 2013CB933703 Fund of the Ministry of Education of the People's Republic of China 20110121110011 PCSIRT IRT13036
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/85053]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Sheng-Lin Cai,Shuo-HuiCao,Yu-BinZheng,et al. Surface chargemodulatedaptasensorinasingleglassconical nanopore[J],2015.
APA	Sheng-Lin Cai.,Shuo-HuiCao.,Yu-BinZheng.,ShuangZhao.,Jin-LeiYang.,...&李耀群.(2015).Surface chargemodulatedaptasensorinasingleglassconical nanopore..
MLA	Sheng-Lin Cai,et al."Surface chargemodulatedaptasensorinasingleglassconical nanopore".(2015).

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