QQ客服 官方微博 反馈留言

	Molecular evolution of paclitaxel biosynthetic genes TS and DBAT of Taxus species
	Hao, D. C. ; Yang, L. ; Huang, B. L. ; Huang BL(黄本立)
	2009
关键词	Adaptive evolution 10-Deacetylbaccatin III-10 beta-O-acetyltransferase Paclitaxel Positive selection Taxadiene synthase Taxus
英文摘要	Evolutionary patterns of sequence divergence were analyzed in genes from the conifer genus Taxus (yew), encoding paclitaxel biosynthetic enzymes taxadiene synthase (TS) and 10-deacetylbaccatin III-10 beta-O-acetyltransferase (DBAT). N-terminal fragments of TS, full-length DBAT and internal transcribed spacer (ITS) were amplified from 15 closely related Taxus species and sequenced. Premature stop codons were not found in TS and DBAT sequences. Codon usage bias was not found, suggesting that synonymous mutations are selectively neutral. TS and DBAT gene trees are not consistent with the ITS tree, where species formed monophyletic clades. In fact, for both genes, alleles were sometimes shared across species and parallel amino acid substitutions were identified. While both TS and DBAT are, overall, under purifying selection, we identified a number of amino acids of TS under positive selection based on inference using maximum likelihood models. Positively selected amino acids in the N-terminal region of TS suggest that this region might be more important for enzyme function than previously thought. Moreover, we identify lineages with significantly elevated rates of amino acid substitution using a genetic algorithm. These findings demonstrate that the pattern of adaptive paclitaxel biosynthetic enzyme evolution can be documented between closely related Taxus species, where species-specific taxane metabolism has evolved recently.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/64481]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Hao, D. C.,Yang, L.,Huang, B. L.,et al. Molecular evolution of paclitaxel biosynthetic genes TS and DBAT of Taxus species[J],2009.
APA	Hao, D. C.,Yang, L.,Huang, B. L.,&黄本立.(2009).Molecular evolution of paclitaxel biosynthetic genes TS and DBAT of Taxus species..
MLA	Hao, D. C.,et al."Molecular evolution of paclitaxel biosynthetic genes TS and DBAT of Taxus species".(2009).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。