A self-referenced nanodosimeter for reaction based ratiometric imaging of hypochlorous acid in living cells

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	A self-referenced nanodosimeter for reaction based ratiometric imaging of hypochlorous acid in living cells
	Wu, Xuanjun ; Li, Zhu ; Yang, Liu ; Han, Jiahuai ; Han, Shoufa ; Han SF(韩守法)
刊名	http://dx.doi.org/10.1039/c2sc21485g
	2013
关键词	MESOPOROUS SILICA NANOPARTICLES FLUORESCENCE PROBE DRUG-DELIVERY MODIFIED PROTEINS TURN-ON MYELOPEROXIDASE OXIDATION GLUTATHIONE GENERATION OXIDANT
英文摘要	Hypochlorous acid (HOCl) is biosynthesized from hydrogen peroxide via catalysis of myeloperoxidase in lysosomes of immunological cells. Despite being harnessed by immune systems against invading pathogens, biogenic HOCl can also damage host tissues and has been associated with a number of diseases. In this edge article, Forster resonance energy transfer based ratiometric imaging of lysosomal HOCl was achieved with silica nanoparticles comprising FITC (donor dye) and a nonfluorescent chemodosimeter which turned into rhodamine (acceptor dye) upon HOCl triggered tandem oxidation and beta-elimination of the doped chemodosimeter. The nanodosimeter exhibited distinct biochemical properties relative to small molecule-based free chemodosimeters, e.g. lysosome-homing specificity and compatibility with aqueous media, enabling facile monitoring of lysosomal HOCl by conventional flow cytometry. The nanoprobe would be of broad utility for studies on in vivo generation and the impact of lysosomal HOCl in living cells or even in animals.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/64290]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Wu, Xuanjun,Li, Zhu,Yang, Liu,et al. A self-referenced nanodosimeter for reaction based ratiometric imaging of hypochlorous acid in living cells[J]. http://dx.doi.org/10.1039/c2sc21485g,2013.
APA	Wu, Xuanjun,Li, Zhu,Yang, Liu,Han, Jiahuai,Han, Shoufa,&韩守法.(2013).A self-referenced nanodosimeter for reaction based ratiometric imaging of hypochlorous acid in living cells.http://dx.doi.org/10.1039/c2sc21485g.
MLA	Wu, Xuanjun,et al."A self-referenced nanodosimeter for reaction based ratiometric imaging of hypochlorous acid in living cells".http://dx.doi.org/10.1039/c2sc21485g (2013).