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	Chromatographic behavior of cadmium in an ion-pair reversed-phase micro HPLC system and its application to the determination of bio-available cadmium in soil samples
	Li, B ; Wang, QQ ; Wang QQ(王秋泉) ; Yan, H ; Yang, LM ; Yang LM(杨利民) ; Huang, BL ; Huang BL(黄本立)
	2003
关键词	micro HPLC bio-available cadmium elemental fractionation
英文摘要	A novel reversed phase ion-pair micro HPLC system with on-line fluorescence detection has been developed systematically and studied for the determination of cadmium in its bio-available fractions of soil samples. In this system, a micro ODS column of 1.0 mm i.d.x150 mm length and a mobile phase containing 6 mmol L-1 8-hydroxyquinoline 5-sulphonic acid (HQS), 3 mmol L-1 cetyltrimethylammonium bromide (CTMABr), 10 mmol L-1 acetic acid-acetate buffer (pH 5.1) as well as 50% acetonitrile at 50 muL min(-1) flow rate were employed to determine cadmium with a 2 muL flow cell through its fluorescence at 518 nm under 338 nm excitation. Furthermore, the composition of Cd-HQS chelate formed on the column was confirmed to be [Cd(HQS)(2)](2-) through a log-log plot method, and then combined with the ion-pair reagent by the electrostatic force under the chromatographic condition proposed. With such a method, the detection limit of cadmium was 8.48 ng mL(-1) (3sigma) with 1 muL sample injection, and the linear range for the determination of cadmium was 30-800 ng mL(-1) (R-2=0.992). This method has been successfully applied to determination of cadmium in its bio-available fractions of BCR-483 and soil samples without interference from other coexistent metal ions. The RSD (n=6) was less than 7.3%. The results were in agreement with the indicative value for BCR-483 and those for the soil samples obtained by ICP-MS with a pretreatment of bis(1,1,3,3-tetramethylbutyl) phosphinic acid extraction.
语种	英语
出版者	SPRINGER-VERLAG BERLIN
内容类型	期刊论文
源URL	[http://dx.doi.org/doi:10.1007/s00216-003-1966-2]
专题	化学化工－已发表论文
推荐引用方式 GB/T 7714	Li, B,Wang, QQ,Wang QQ,et al. Chromatographic behavior of cadmium in an ion-pair reversed-phase micro HPLC system and its application to the determination of bio-available cadmium in soil samples[J],2003.
APA	Li, B.,Wang, QQ.,王秋泉.,Yan, H.,Yang, LM.,...&黄本立.(2003).Chromatographic behavior of cadmium in an ion-pair reversed-phase micro HPLC system and its application to the determination of bio-available cadmium in soil samples..
MLA	Li, B,et al."Chromatographic behavior of cadmium in an ion-pair reversed-phase micro HPLC system and its application to the determination of bio-available cadmium in soil samples".(2003).

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