| 核苷酸超滤纯化技术的优化; Optimization of ultrafiltration technology for purification of nucleotide | |
| 谢全灵 ; 夏海平 ; 刘嘉 ; 丁马太 ; 黄桂辉 ; 何旭敏 ; 蓝伟光 | |
| 2005-08 | |
| 关键词 | RNA 核苷酸 超滤 膜分离 nucleotide ultrafiltratioin membrane separation |
| 英文摘要 | 从膜通量、蛋白去除率、核苷酸收率、膜污染和膜清洗等方面考察了5种不同型号的Ultra-floTM超滤膜过滤RNA酶解液的效果。实验表明3#(MWCO30 kD,CA)Ultra-floTM超滤膜同时具有高收率(96.4%)、高蛋白去除率(81.3%)、高膜通量(65.6L.m-2.h-1)、易清洗(水通量恢复99%)的优点,不仅可以替代通常使用的中空纤维膜,且通量大、污染小、易清洗,使该过程得到优化。The filt ration performances of enzymatic RNA degradation liquid wit h five different Ult ra2flo TM ult ra2 filt ration membranes have been st udied by comparing flux , protein rejection , nucleotide yield , membrane foul2 ing and membrane cleaning. The result s show t hat 3 # Ult ra2flo TM ult rafilt ration membrane (MWCO 30kD) was t he optimum , having a high nucleotide yield (96. 4 %) , high protein rejection (81. 3 %) , high flux (65. 6L ·m- 1 ·h - 1 ) , high recovery ratio of water flux (99 %) . This characteristic of high flux , low fouling and easy cleaning leads us to conclude t hat 3 # Ult ra2flo TM ult rafilt ration membrane was a good candidate for replacing t he com2 monly used hollow fiber membrane to achieve an optimized filt ration of enzymatic RNA degradation liquid.; 福建省科技重点资助项目(2000-I-015);福建省青年科技人才创新资助项目(2001J065). |
| 语种 | 中文 |
| 出版者 | 重庆仪表材料研究所 |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/3078]  |
| 专题 | 化学化工－已发表论文 |
| 推荐引用方式 GB/T 7714 | 谢全灵,夏海平,刘嘉,等. 核苷酸超滤纯化技术的优化, Optimization of ultrafiltration technology for purification of nucleotide[J],2005. |
| APA | 谢全灵.,夏海平.,刘嘉.,丁马太.,黄桂辉.,...&蓝伟光.(2005).核苷酸超滤纯化技术的优化.. |
| MLA | 谢全灵,et al."核苷酸超滤纯化技术的优化".(2005). |
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