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	杂色鲍后消化道生长相关基因HdEGF1的研究; A Study of HdEGF1,an EGF-related Gene in the Lower Digestive Tract from Small Abalone Haliotis diversicolor
	张洁 ; 陈志森 ; 陈军 ; 柯才焕 ; 游伟伟
	2013-07-28
关键词	杂色鲍 附着变态 表皮生长因子相关蛋白 HdEGF1基因 消化道末端 Haliotis diversicolor settlement and metamorphosis epidermal growth factor(EGF)-related protein HdEGF1 gene lower digestive tract
英文摘要	附着变态是许多海洋无脊椎动物幼体生长发育的一个必经过程,是幼体性状消失而成体性状展开的关键阶段.报道了与附着变态密切相关的杂色鲍HdEgf1基因的克隆、序列分析及其时空表达模式.运用CdnA末端快速扩增技术(rACE)克隆了HdEgf1基因全长CdnA序列.该基因全长1 239bP,含1个343个氨基酸残基的开放阅读框(Orf),推测的蛋白序列包含3个CA2+结合类表皮生长因子(Egf-CA)结构域和1个血管性血友病因子(VWfA)结构域.通过序列相似性比较,确定其为新型表皮生长因子1(Egf1)相关蛋白.荧光定量PCr(QPCr)结果显示HdEgf1基因在幼体附着后的表达量比附着前任一时期均高19倍以上;全胚原位杂交(WMISH)结果显示HdEgf1基因集中表达在变态后幼体后消化道的表皮细胞.HdEgf1基因的这种时空表达模式表明HdEgf1受严格的时空调控,直接参与了附着后杂色鲍幼体后消化道的生长和细胞分化.推测HdEgf1蛋白与某未知的VWfA-结合蛋白一道调控着杂色鲍幼体后消化道的空间走向.因此,HdEgf1蛋白很可能在后肠的形态建成中起着非常核心的作用.本研究为贝类肠道形态建成机制的深入研究提供了切入点,HdEgf1基因可以作为重要的标志分子,用于深入研究肠道形态建成过程中的信号通路、细胞分化和细胞迁移.; Settlement and metamorphosis are indispensable processes in the development of most marine invertebrate larvae,which are critical stages directly related to the larvae developing to adult.The present study identified the full-length cDNA sequence of a HdEGF1 gene from Haliotis diversicolor.the 1 239 bp full-length cDNA sequence was obtained through the technology of Rapid Amplification of cDNA Ends(RACE).This cDNA encodes a novel epidermal growth factor(EGF) related protein with 343 amino acid residues that contains three calcium-binding EGF-like domains(EGF-CA) and a von willebrand factor type A domain(vWFA).Using quantitative PCR(qPCR) approach,the expression of the HdEGF1 gene was found to be 19-fold higher at metamorphosis stage than at any pre-settlement stages.In addition,the result of whole mount in situ hybridization(WMISH) indicated that the mRNAs of the HdEGF1 gene were found to be accumulated in the epidermal cells of the primitive hindgut of postlarvae.The vWFA domain analyses showed that the HdEGF1 protein might control the spatial distribution of the lower digestive tract via interaction with vWFA-binding proteins.The differences of HdEGF1 gene expression patterns before and after the settlement indicate that the development of the digestive tract downstream tissues is subjected to the strict regulation of settlement and metamorphosis.In conclusion,the HdEGF1 gene may play central roles in the development of lower digestive tract.; 国家自然科学基金项目(40976093); 国家高技术研究发展计划(863)项目(2012AA10A412)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/104670]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	张洁,陈志森,陈军,等. 杂色鲍后消化道生长相关基因HdEGF1的研究, A Study of HdEGF1,an EGF-related Gene in the Lower Digestive Tract from Small Abalone Haliotis diversicolor[J],2013.
APA	张洁,陈志森,陈军,柯才焕,&游伟伟.(2013).杂色鲍后消化道生长相关基因HdEGF1的研究..
MLA	张洁,et al."杂色鲍后消化道生长相关基因HdEGF1的研究".(2013).

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