Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery

CORC > 厦门大学 > 海洋环境－已发表论文

	Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery
	Dong, Hong-Po ; Williams, Ernest ; Wang, Da-zhi ; Xie, Zhang-Xian ; Hsia, Ru-ching ; Jenck, Alizee ; Halden, Rolf ; Li, Jing ; Chen, Feng ; Place, Allen R. ; Dong HP(董宏坡) ; Wang DZ(王大志)
刊名	http://dx.doi.org/10.1104/pp.113.214320
	2013
关键词	CHLAMYDOMONAS-REINHARDTII LIPID-ACCUMULATION BIOFUEL PRODUCTION GROWTH METABOLISM MICROALGAE SEAWATER ALGAE PURIFICATION DEPRIVATION
英文摘要	National Basic Research Program of China (973 Program) [2010CB428702]; National Natural Science Foundation of China [41206126]; University of Maryland Center for Environmental Sciences [4755]; Institute of Marine and Environmental Technology [13106]; The Nannochloropsis genus contains oleaginous microalgae that have served as model systems for developing renewable biodiesel. Recent genomic and transcriptomic studies on Nannochloropsis species have provided insights into the regulation of lipid production in response to nitrogen stress. Previous studies have focused on the responses of Nannochloropsis species to short-term nitrogen stress, but the effect of long-term nitrogen deprivation remains largely unknown. In this study, physiological and proteomic approaches were combined to understand the mechanisms by which Nannochloropsis oceanica IMET1 is able to endure long-term nitrate deprivation and its ability to recover homeostasis when nitrogen is amended. Changes of the proteome during chronic nitrogen starvation espoused the physiological changes observed, and there was a general trend toward recycling nitrogen and storage of lipids. This was evidenced by a global down-regulation of protein expression, a retained expression of proteins involved in glycolysis and the synthesis of fatty acids, as well as an up-regulation of enzymes used in nitrogen scavenging and protein turnover. Also, lipid accumulation and autophagy of plastids may play a key role in maintaining cell vitality. Following the addition of nitrogen, there were proteomic changes and metabolic changes observed within 24 h, which resulted in a return of the culture to steady state within 4 d. These results demonstrate the ability of N. oceanica IMET1 to recover from long periods of nitrate deprivation without apparent detriment to the culture and provide proteomic markers for genetic modification.
语种	英语
出版者	AMER SOC PLANT BIOLOGISTS
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/88020]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Dong, Hong-Po,Williams, Ernest,Wang, Da-zhi,et al. Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery[J]. http://dx.doi.org/10.1104/pp.113.214320,2013.
APA	Dong, Hong-Po.,Williams, Ernest.,Wang, Da-zhi.,Xie, Zhang-Xian.,Hsia, Ru-ching.,...&王大志.(2013).Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery.http://dx.doi.org/10.1104/pp.113.214320.
MLA	Dong, Hong-Po,et al."Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery".http://dx.doi.org/10.1104/pp.113.214320 (2013).