Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi

CORC > 厦门大学 > 海洋环境－已发表论文

	Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi
	Qin Yingxue ; Yan Qingpi ; Su Yongquan ; Li Haiping ; Zou Wenzheng ; Su YQ(苏永全)
刊名	http://dx.doi.org/10.1007/s13131-013-0341-1
	2013
关键词	HELICOBACTER-PYLORI SKIN MUCUS MOTILITY BACTERIA ANGUILLARUM IDENTIFICATION RESISTANCE FLAGELLIN CHOLERAE ADHESION
英文摘要	National Natural Science Foundation of China [31272699, 41176115]; National Department Public Benefit Research Foundation of China [200903029]; Natural Science Foundation of Fujian Province [2011J06014]; National Hi-Tech Research and Development Program of China (863 Program) [2007AA09Z115]; Chemotactic motility is involved in the virulence of many pathogenic bacteria. In order to understand the role of chemotactic motility of Vibrio harveyi in cellular processes and virulence, mini-Tn10/Kan transposon-induced mutants with deficient chemotactic motility were constructed, screened, and identified. Sequence analysis revealed that the 465-bp fragment (Gen Bank accession number HM630274) flanking the transposon insertion site in mutant TS-CM1 had the highest identity (96.9%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (91.8%) with the pgk gene of V. parahaemolyticus RIMD 2210633. In another mutant, TS-CM2, 356 bp of transposon-flanking sequence (Gen Bank accession number HM630275) also showed the highest identity (94.6%) with a hypothetical protein gene of V. harveyi ATCC BAA-1116 and the second-highest identity (92.4%) with the flaB gene of V. alginolyticus HY9901. Studies on virulence-related biological characteristics such as growth, motility, adhesion, and infectivity of themutants showed that disruption of either the flagellin gene or energy metabolism gene led to subsequent loss of chemotactic motility and changes in growth, motility, adhesion, and virulence of the pathogenic V. harveyi. Hence, the flagellin gene and crucial energy metabolism gene played an important role in the chemotactic motility of V. harveyi.
语种	英语
出版者	SPRINGER
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/87991]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Qin Yingxue,Yan Qingpi,Su Yongquan,et al. Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi[J]. http://dx.doi.org/10.1007/s13131-013-0341-1,2013.
APA	Qin Yingxue,Yan Qingpi,Su Yongquan,Li Haiping,Zou Wenzheng,&苏永全.(2013).Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi.http://dx.doi.org/10.1007/s13131-013-0341-1.
MLA	Qin Yingxue,et al."Disruption of chemotaxis-related genes affects multiple cellular processes and the virulence of pathogenic Vibrio harveyi".http://dx.doi.org/10.1007/s13131-013-0341-1 (2013).