Proteomic Responses of Roseobacter litoralis OCh149 to Starvation and Light Regimen

CORC > 厦门大学 > 海洋环境－已发表论文

	Proteomic Responses of Roseobacter litoralis OCh149 to Starvation and Light Regimen
	Zong, Rui ; Jiao, Nianzhi ; Jiao NZ(焦念志)
刊名	http://dx.doi.org/10.1264/jsme2.ME12029
	2012-12-07
关键词	DISSOLVED ORGANIC-CARBON RHODOBACTER-SPHAEROIDES MARINE ALPHAPROTEOBACTERIA PHAEOBACTER-GALLAECIENSIS POLYHYDROXYALKANOATE PHA DINOROSEOBACTER-SHIBAE BACTERIOCHLOROPHYLL-A GENOME SEQUENCE METABOLISM BACTERIA
英文摘要	NSFC [91028001, 41076063]; SOA [201105021]; Roseobacter litoralis OCh149 is a type strain of aerobic anoxygenic phototrophic bacteria in marine Roseobacter clade. Its full genome has been sequenced; however, proteomic research, which will give deeper insights into the environmental stimuli on gene expression networks, has yet to be performed. In the present study, a proteomic approach was employed to analyze the status of R. litoralis OCh149 in carbon starvation during the stationary phase and its responses to a dark/light regimen (12 h: 12 h) in both exponential and stationary phases. LC-MS/MS-based analysis of highly abundant proteins under carbon starvation revealed that proteins involved in transport, the transcription/translation process and carbohydrate metabolism were the major functional categories, while poly-beta-hydroxyalkanoate (PHA), previously accumulated in cells, was remobilized after stress. Glucose, as the sole carbon source in the defined medium, was broken down by Entner-Doudoroff and reductive pentose phosphate (PP) pathways. Carbohydrate catabolism-related proteins were down-regulated under light regardless of the growth phase, probably due to inhibition of respiration by light. In contrast, responses of amino acid metabolisms to light regimen varied among different proteins during growth phases depending on cellular requirements for proliferation, growth or survival. Fluorescence induction and relaxation measurements suggested that functional absorption cross-sections of the photosynthetic complexes decreased during the dark period and always recovered to about the previous level during the light period. Although the photosynthetic genes in R. litoralis OCh149 are located on the plasmid, these data indicate the regulatory mechanism of photoheterotroph metabolism by both carbon and light availability.
语种	英语
出版者	MICROBES ENVIRON
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/87850]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Zong, Rui,Jiao, Nianzhi,Jiao NZ. Proteomic Responses of Roseobacter litoralis OCh149 to Starvation and Light Regimen[J]. http://dx.doi.org/10.1264/jsme2.ME12029,2012.
APA	Zong, Rui,Jiao, Nianzhi,&焦念志.(2012).Proteomic Responses of Roseobacter litoralis OCh149 to Starvation and Light Regimen.http://dx.doi.org/10.1264/jsme2.ME12029.
MLA	Zong, Rui,et al."Proteomic Responses of Roseobacter litoralis OCh149 to Starvation and Light Regimen".http://dx.doi.org/10.1264/jsme2.ME12029 (2012).