A marine bacterium producing protein with algicidal activity against Alexandrium tamarense

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	A marine bacterium producing protein with algicidal activity against Alexandrium tamarense
	Wang, Binxiang ; Yang, XiaoRu ; Lu, Jinglin ; Zhou, Yanyan ; Su, Jianqiang ; Tian, Yun ; Zhang, Jun ; Wang, Guizhong ; Zheng, Tianling ; Wang GZ(王桂忠)
刊名	http://dx.doi.org/10.1016/j.hal.2011.10.006
	2012-01
关键词	TIDE CAUSING ALGA RED-TIDE TOXIC DINOFLAGELLATE BLOOMS SPP.
英文摘要	National Natural Science Foundation of China [40930847, 31070442, 40876061]; National High Technology Research and Development Program of China [2008AA09Z408]; Program for Changjiang Scholars and Innovative Research Team in University [40821063]; Interactions between bacteria and harmful algal bloom (HAB) species have been acknowledged as an important factor of regulating the population of these algae. In the study, two strains of algicidal bacteria, DHQ25 and DHY3, were screened out because of their probably secreting algicidal proteins against axenic Alexandrium tamarense. Molecular characterization classified them to the gamma-proteobacteria subclass and to the genus Vibrio and Pseudoalteromonas, respectively. After centrifugation and ultrafiltration, chromatography of the cultural supernatants of DHQ25 revealed 8 peaks by HPLC. SDS-PAGE and Native PAGE determination showed that peak 7 to be a monoband peak. Both xenic and axenic culture of A. tamarense were susceptible to the purified protein (short for P7 below) indicated by algicidal activity assay. Observation of algicidal process demonstrated that algal cells were lysed and cellular substances were released under visual fields of microscope. P7 proved to be a challenge controller of A. tamarense by the above characterizations of algicidal activity assaying and algicidal process. This is the first report of a protein algicidal to the toxic dinoflagellate A. tamarense. The findings increase our knowledge of bacterial-algal interactions and the role of bacteria during controlling HABs. (C) 2011 Elsevier B.V. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/60627]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Wang, Binxiang,Yang, XiaoRu,Lu, Jinglin,et al. A marine bacterium producing protein with algicidal activity against Alexandrium tamarense[J]. http://dx.doi.org/10.1016/j.hal.2011.10.006,2012.
APA	Wang, Binxiang.,Yang, XiaoRu.,Lu, Jinglin.,Zhou, Yanyan.,Su, Jianqiang.,...&王桂忠.(2012).A marine bacterium producing protein with algicidal activity against Alexandrium tamarense.http://dx.doi.org/10.1016/j.hal.2011.10.006.
MLA	Wang, Binxiang,et al."A marine bacterium producing protein with algicidal activity against Alexandrium tamarense".http://dx.doi.org/10.1016/j.hal.2011.10.006 (2012).