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	Characteristics and Kinetics of Iron Releasefrom the Ferritin under the EGCG reduction
	Ji, Xuetao ; Huang, Lin ; Lin, Qing ; Huang, Heqing ; Huang HQ(黄河清)
	2012-04
关键词	Liver ferritin of Dasyatis akajei (DALF) EGCG Reduction Fluorescence spectrometry CD spectrometry Kinetic of iron release
英文摘要	The mechanism of iron release from ferritin in vivo is still unclear even though it represents a key step of the metabolism of iron in vivo. Here, both interaction intensity and binding stability between epigallocatechin gallate (EGCG) from tea and liver ferritin of Dasyatis akajei (DALF) were investigated using UV-visible, fluorescence and circular dichroism (CD) spectrometry, respectively. The results indicated that EGCG could reduce the iron within the ferritin shell directly in the absence of chemical reducers such as Na2S2O4, but this process was strictly pH-dependent, and the rate of iron release is faster at low pH than at high pH. The kinetic study of iron release showed that this process fitted the law of zero order reaction, which differed from that of first order reaction by various chemical reducers such as Vitamin C. In addition, Both fluorescence and CD spectrometry were further used to study the reduction mechanism of iron release in vitro, showing that there was a slight conformation change of the ferritin shell during EGCG reduction because of a complex formation of DALF-EGCG. It appears that chemical reducers with large molecular sizes reduce the iron across the protein shell by the way of an electron transfer pathway (ETP). A novel pathway for iron release from DALF with EGCG reduction is suggested to explain for a reductive route of iron metabolism by biological reducers in vivo.; State Natural Science Fund, China [30870515]
语种	英语
出版者	HUMANA PRESS INC
内容类型	期刊论文
源URL	[http://dx.doi.org/10.1007/s12011-011-9225-4]
专题	海洋环境－已发表论文
推荐引用方式 GB/T 7714	Ji, Xuetao,Huang, Lin,Lin, Qing,et al. Characteristics and Kinetics of Iron Releasefrom the Ferritin under the EGCG reduction[J],2012.
APA	Ji, Xuetao,Huang, Lin,Lin, Qing,Huang, Heqing,&黄河清.(2012).Characteristics and Kinetics of Iron Releasefrom the Ferritin under the EGCG reduction..
MLA	Ji, Xuetao,et al."Characteristics and Kinetics of Iron Releasefrom the Ferritin under the EGCG reduction".(2012).

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