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	榄香烯乳逆转肺腺癌A549/DDP细胞耐药性及可能作用机制的研究; The efect of elemene reversing the multidurg resistance of A549/DDP lung cancer cells
	姚成才 ; 涂远荣 ; 杜好信 ; 姜杰 ; 叶社房 ; 张义 ; 陈隽鹏
	2013-12-25
关键词	肺肿瘤 抗药性 肿瘤 细胞凋亡 榄香烯 A549/DDP细胞 Lung neoplasms Drug resietance neoplasms Apoptosis Elemene A549/DDP cells
英文摘要	目的 :探讨榄香烯乳(ElEMEnE)逆转人肺腺癌耐顺铂(CISPlATIn,ddP)细胞A549/ddP的耐药性及可能的作用机制。方法 :采用MTT法检测榄香烯乳单药作用时的细胞毒作用及与ddP联合作用对A549/ddP细胞耐药的逆转作用;HOECHST 33342法进行细胞核染色,荧光显微镜下观察细胞形态的改变;fCM检测细胞凋亡率;蛋白质印迹法检测细胞质中细胞色素C(CyTOCHrOME C,CyT C)、PrO-CASPASE-3、CASPASE-3和抗凋亡因子b细胞淋巴瘤-2(b CEll lyMPHOMA-2,bCl-2)家族蛋白表达的情况。结果 :不同浓度榄香烯乳对A549/ddP细胞均有一定的抑制作用,呈时间-剂量依赖性效应,联合ddP能提高耐药细胞株A549/ddP对ddP的敏感性而逆转其耐药性。HOECHST 33342法和fCM法检测结果显示,榄香烯乳联合ddP能更大程度上诱导A549/ddP细胞的凋亡,提高耐药细胞的凋亡率,同时还上调A549/ddP细胞质中CyT C、CASPASE-3和bAd蛋白的表达,而下调PrO-CASPASE-3和bCl-2蛋白的表达。结论 :榄香烯乳能逆转A549/ddP细胞耐药性可能与其损伤线粒体膜,使线粒体释放CyT C到细胞质、同时活化CASPASE-3、上调促凋亡蛋白bAd和下调抗凋亡蛋白bCl-2表达以启动凋亡路径有关。; Objective: To investigate the effect of elemene on the drug resistance of cisplatin(DDP)- resistant human lung adenocarcinoma A549/DDP cells, and its possible mechanism.Methods: The growth inhibition of A549/DDP cells treated with elemene alone or combined with different concentrations of DDP was detected by MTT assay.The morphological changes of apoptosis of A549/DDP cells treated with different concentrations of elemene(20 and 40 μg/mL) for 24 h were observed by Hoechst 33342 staing under fluorescence a microscope.The apoptosis rate of A549/DDP cells treated with different concentrations of elemene(20 and 40 μg/mL) after 24 h were detected by FCM(flow cytometry).The expressions of cytochrome C, pro-caspase-3, caspase-3 and the Bcl-2 family proteins were measured by Western blotting analysis.Results: MTT result showed that different concentrations elemene could inhibit the proliferation of A549/DDP cells in a time- and dose- dependent manner.Intriguingly, elemene plus DDP enhanced the sensitivity of A549/ DDP cells to DDP and reversed the resistance of A549/DDP cells.Elemene was also a strong inducer of apoptosis in this model system, and a synergistic effect on induction of cell death was observed when the tumor cells were treated with both agents.The result showed that elemene could enhance A549/DDP cell apoptosis.Furthermore, the combination of elemene and DDP also enhanced the protein expressions of cytochrome C, caspase-3 and Bad, and reduced the protein levels of Bcl-2 and pro-caspase-3 in the A549/DDP cells.Conclusion: The reversal of the multi-drug resistance of A549/DDP cell line by elemene may result from its effect on induction of apoptosis.Elemene treatment can impaire mitochondrial membrane, release cytochrome C into cytoplasm, activate caspase-3,up-regulate pro-apoptotic protein Bad and down-regulate anti-apoptotic protein Bcl-2, and finally caused apoptosis.; 福建省自然科学基金面上项目(编号:2010D014)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/98427]
专题	材料学院－已发表论文
推荐引用方式 GB/T 7714	姚成才,涂远荣,杜好信,等. 榄香烯乳逆转肺腺癌A549/DDP细胞耐药性及可能作用机制的研究, The efect of elemene reversing the multidurg resistance of A549/DDP lung cancer cells[J],2013.
APA	姚成才.,涂远荣.,杜好信.,姜杰.,叶社房.,...&陈隽鹏.(2013).榄香烯乳逆转肺腺癌A549/DDP细胞耐药性及可能作用机制的研究..
MLA	姚成才,et al."榄香烯乳逆转肺腺癌A549/DDP细胞耐药性及可能作用机制的研究".(2013).

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