手性钌(Ⅱ)配合物对DNA的立体选择性断裂

CORC > 深圳大学

	手性钌(Ⅱ)配合物对DNA的立体选择性断裂
	王芳 ; 张黔玲 ; 刘剑洪 ; 张培新 ; 刘毅 ; 任祥忠 ; 刘建忠 ; 计亮年
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=GDXH200411006&dbname=CJFQ2004
	2012-04-27 ; 2012-04-27
关键词	手性钌(Ⅱ)配合物 DNA 插入断裂
中文摘要	Two enantiomerically pure polypyridyl ruthenium(Ⅱ) complexes Δ- and Λ-[Ru(bpy) 2HPIP](PF 6) 2{HPIP=2-(2-hydroxyphenyl)imidazo[4,5-f][1,10]phenanthroline} were synthesized and characterized. DNA-binding studies indicated that both enantiomers bound to calf thymus DNA by intercalation, the Δ- enantiomer exhibited a stronger binding affinity than the Λ- enantiomer. Upon irradiation at 302 nm, both enantiomers were found to promote cleavage of plasmid pBR 322 DNA from the supercoiled form Ⅰ to the open circular form Ⅱ, but the Δ-enantiomer exhibited a higher cleaving efficiency for DNA due to the different binding affinities to DNA. The cleaving mechanisms for Δ- and Λ-[Ru(bpy) 2HPIP] 2+ were identical, the hydroxyl radical(OH ·) was likely to be the reactive specie responsible for the cleavage of plasmid pBR 322, and the photoreduction of Ru(Ⅱ) complex with concomitant hydroxide oxidation was the important step in the DNA cleavage reaction.
语种	中文
其他责任者	深圳大学师范学院化学与生物学系 ; 深圳大学师范学院物理系 ; 中山大学教育部基因工程重点实验室 ; 中山大学教育部基因工程重点实验室深圳518060 ; 深圳518060 ; 广州510275
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/244041/411]
专题	深圳大学
推荐引用方式 GB/T 7714	王芳,张黔玲,刘剑洪,等. 手性钌(Ⅱ)配合物对DNA的立体选择性断裂[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=GDXH200411006&dbname=CJFQ2004,2012, 2012.
APA	王芳.,张黔玲.,刘剑洪.,张培新.,刘毅.,...&计亮年.(2012).手性钌(Ⅱ)配合物对DNA的立体选择性断裂.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=GDXH200411006&dbname=CJFQ2004.
MLA	王芳,et al."手性钌(Ⅱ)配合物对DNA的立体选择性断裂".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=GDXH200411006&dbname=CJFQ2004 (2012).