反相高效液相色谱法同时测定九种食品添加剂

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	反相高效液相色谱法同时测定九种食品添加剂
	吴光斌 ; 吕禹泽 ; 陈发河
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spkx200611113&dbcode=CJFQ&dbname=CJFQ2006
	2012-06-05 ; 2012-06-05
关键词	食品食品添加剂高效液相色谱法 food food additives high performance liquid chromatography . . TS207.3
其他题名	Simultaneous Determination of Night Food Additives by Reversed-Phase High Performance Liquid Chromatography
中文摘要	采用反相高效液相色谱法,一次进样,同时测定食品中常见的9种食品添加剂:苯甲酸、山梨酸、糖精钠、安赛密、柠檬黄、日落黄、胭脂红、苋菜红、咖啡因。实验采用Nova-PakC18柱(3.9mm×150mm,4μm,Waters)为分离柱,以20mmol/L乙酸铵(pH6.8)-甲醇为流动相(梯度洗脱:甲醇:5%5min;5%～30%,12.5%/min;30%继续3min;30%～5%,25%/min),采用紫外检测器在234nm下进行检测。整个分析过程在10min内完成。其平均加标回收率为77.0%～108.5%,相对标准偏差小于5%。该方法结果准确,适用于食品常规质量检测。; A novel method is proposed for the simultaneous separation and determination of night food additives,saccharin sodium,acesulfame potassium,benzoic acid,sorbic acid,carmine,amaranth,tartrazine,sunset yellow and caffeine by reversed- phase high performance liquid chromatography. The separation was achieved within 10 min by using a Nova-Pak reverse phase C18 column (150mm×3.9mm,4μm,Waters Inc) with 20mmol/L NH4Ac (pH6.8)-methanol as mobile phase (gradient elution: methanol: 5% 5min; 5%～30%,12.5%/min; 30% continued 3min; 30%～5%,25%/min),and lastly detected at 234nm through a UV detector. The average recoveries for real samples ranged from 77.0% to 108.5%.; 【作者单位】集美大学生物工程学院；集美大学生物工程学院福建厦门361021；福建厦门361021；【作者英文名】WU Guang-bin,LU Yu-ze,CHEN Fa-he* (School of Bioengineering,Jimei University,Xiamen 361021,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18648]
专题	集美大学
推荐引用方式 GB/T 7714	吴光斌,吕禹泽,陈发河. 反相高效液相色谱法同时测定九种食品添加剂[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spkx200611113&dbcode=CJFQ&dbname=CJFQ2006,2012, 2012.
APA	吴光斌,吕禹泽,&陈发河.(2012).反相高效液相色谱法同时测定九种食品添加剂.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spkx200611113&dbcode=CJFQ&dbname=CJFQ2006.
MLA	吴光斌,et al."反相高效液相色谱法同时测定九种食品添加剂".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spkx200611113&dbcode=CJFQ&dbname=CJFQ2006 (2012).