红毛菜藻红蛋白的提取及稳定性研究

CORC > 集美大学

	红毛菜藻红蛋白的提取及稳定性研究
	吕彩真 ; 欧光南
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200201003&dbcode=CJFQ&dbname=CJFQ2002
	2012-06-05 ; 2012-06-05
关键词	红毛菜藻红蛋白冻融法稳定性光解作用 Bangia fusco-purpurea phycoerythin freezing and thawing stability photolysis mechanism O629
其他题名	Extraction and Stability of Phycoerythrin(PE) in Bangia fusco-purpurea
中文摘要	用多种提取方法提取红毛菜 (Bangiafusco purpurea)藻红蛋白并对藻红蛋白粗提液的稳定性进行了初步研究 .冻融法提取藻红蛋白的得率较高 ;温度、pH、铜离子、铁离子及光照对藻红蛋白稳定性有很大影响 ,藻红蛋白较适宜的保存条件为 :低温 (5℃左右 )、pH 5 .7、避光 .根据实验结果 ,提出了藻胆蛋白光解反应的可能机理 .; The phycoerythrin (PE) in the Bangia fusco-purpurea was extracted more effectively through repeated freezing and thawing (three times) in the presence of 0.1 mol/L phosphate buffer than by any other ways.The stability of the PE crude extract was greatly affected by temperature,pH,copper ion,ferrous ion,and light.The optimum preserving circumstance for the crude extract is:low temperature (about 5℃),pH 5.7 and away from light.A probable photolysis mechanism of phycobiliproteins was proposed.; 【作者单位】厦门金利华有限公司；集美大学生物工程学院福建厦门361009；福建厦门361021；【作者英文名】LU Cai-zhen 1,OU Guang-nan 2 (1.Kamliway Enterprise Co.Ltd.,Xiamen 361009,China; 2.School of Biotechnology,Jimei University,Xiamen 361021,China)
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/18399]
专题	集美大学
推荐引用方式 GB/T 7714	吕彩真,欧光南. 红毛菜藻红蛋白的提取及稳定性研究[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200201003&dbcode=CJFQ&dbname=CJFQ2002,2012, 2012.
APA	吕彩真,&欧光南.(2012).红毛菜藻红蛋白的提取及稳定性研究.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200201003&dbcode=CJFQ&dbname=CJFQ2002.
MLA	吕彩真,et al."红毛菜藻红蛋白的提取及稳定性研究".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=jmxz200201003&dbcode=CJFQ&dbname=CJFQ2002 (2012).