藻胆蛋白的提取纯化研究进展

CORC > 集美大学

	藻胆蛋白的提取纯化研究进展
	郑江
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spkx200211040&dbcode=CJFQ&dbname=CJFQ2002
	2012-06-05 ; 2012-06-05
关键词	藻胆蛋白提取纯化 Phycobiliproteins Extraction Purification O629
其他题名	Recent Research on Extration and Purification of Phycobiliproteins
中文摘要	本文从提取原料、细胞破碎方法、粗提和纯化方法等几方面对近期有关藻胆蛋白的提取纯化研究做一介绍。常用的提取原料有螺旋藻、多管藻、条斑紫菜等;细胞破碎方法有反复冻融法、化学试剂处理法等五种;粗提方法主要有盐析法等四种;而常用的纯化方法有羟基磷灰石柱层析法、DEAE-纤维素离子交换柱法和葡聚糖凝胶柱层析法等三种。实际应用中为获得较好的提取纯化效果,经常混合使用多种细胞破碎方法和多种纯化方法。; In this paper, the recent researches for phycobiliproteins on extraction and purification were introduced in the aspects of extraction of some raw materials, crushing cell methods, crude extraction methods and purification methods. The usual extracting materials were concluded as Spirulina, Polysiphonia urceolasa, Por-phyra yezoensis etc. There were 5 ways to cruch cell such as repeated freezing and thawing method and chemical reagent method. And there had 4 crude extraction methods such as the salting - out method to extract the crude phycobiliproteins. Hydroxylapatite column chromatography, DEAE - celluose column chromatography and Gel column chromatography were the usual purification methods. To get better results in the practice, diversified methods were often used.; 【作者单位】集美大学水产学院厦门　361021；【作者英文名】Zheng Jiang
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12460]
专题	集美大学
推荐引用方式 GB/T 7714	郑江. 藻胆蛋白的提取纯化研究进展[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spkx200211040&dbcode=CJFQ&dbname=CJFQ2002,2012, 2012.
APA	郑江.(2012).藻胆蛋白的提取纯化研究进展.http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spkx200211040&dbcode=CJFQ&dbname=CJFQ2002.
MLA	郑江."藻胆蛋白的提取纯化研究进展".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=spkx200211040&dbcode=CJFQ&dbname=CJFQ2002 (2012).