真鲷仔鱼用微粒子饲料中肽酶与大豆卵磷脂添加效果的研究(英文)

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	真鲷仔鱼用微粒子饲料中肽酶与大豆卵磷脂添加效果的研究(英文)
	王秋荣 ; 竹内俊郎 ; 古板博文
刊名	http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sswx200606017&dbcode=CJFQ&dbname=CJFQ2006
	2012-06-05 ; 2012-06-05
关键词	真鲷仔鱼微粒子饲料肽酶大豆卵磷脂 Pagrus major larvae Microparticle diet Peptidase Soybean lecithin S963
其他题名	STUDIES ON THE ROLE OF PEPTIDASE AND POWDER SOYBEAN LECITHIN IN MICROPARTICLE DIETS FOR RED SEA BREAM PAGRUS MAJOR LARVAE
中文摘要	本研究以酪蛋白分解物为蛋白源配制三种微粒子饲料MD-S、MD-T和MD-U对真鲷开口仔鱼进行饲养试验。以MD-S的配方为基准,MD-T采用粉状大豆卵磷脂和麸质代替液状大豆卵磷脂;MD-U则另外添加0.1%的肽酶。结果表明,微粒子饲料在水中浸泡15min后,MD-T的溶出率(35.5%)低于MD-S(46.8%)和MD-U(45.8%);实验结束时(20日龄),仔鱼的成活率以生物饵料(轮虫)组为最高(86.3%),其次是MD-T组为20.7%,显著高于(P<0.05)MD-S组(13.3%)和MD-U组(13.6%);生物饵料组的仔鱼全长(6.14±0.49mm)显著大于微粒子饲料组(4.23±0.30mm~4.46±0.30mm),各微粒子饲料组之间仔鱼的全长并不存在显著差异(P>0.05)。在孵化后第12d,微粒子饲料组的仔鱼肠上皮细胞发育良好,但至孵化后第18d,仔鱼肠上皮细胞大部分萎缩、并发生脱落。鱼体的蛋白质、DNA与RNA日间增长率微粒子饲料MD-T组高于MD-S和MD-U组,但都低于生物饵料组。由此可见,微粒子饲料中添加肽酶并无助真鲷仔鱼对其消化吸收;可是,使用粉状大豆卵磷脂与麸质代替液状卵磷脂能增强微粒子饲料的黏合性,可减少其营养成分的溶出率,从而提高微粒子饲料的饲育效果。; This study investigates the effects of exogenous enzyme supplementation on the digestibility of microparticle diets (MD). Powder soybean lecithin and gluten were used instead of paste soybean lecithin for evaluating its effect on the leaching rate in MD. Three kinds of MD (MD-T; MD-S; MD-U) were prepared. Based on formulation of MD-S,MD-T included powder soybean lecithin instead of paste soybean lecithin and gluten as a binder. MD-U was supplied with 0.1% of peptidase. The other ingredients were the same in the three diets. Within the first 15 minutes of submersion in water, the leaching rate for MD-T (35.5%) was lower than for MD-S (46.8%) and MD-U (45.8%). The first feeding red sea bream larvae were fed the three above mentioned MD and live food (LF) as a control. The trial lasted until 20 days after hatching. Final survival rate was highest in LF treatment (86.3%), followed by MD-T (20.7%) treatment, which was significantly higher (P<0.05) than the results for MD-S (13.3%) and MD-U (13.6%) treatments. Final total length (TL) of larvae fed LF was with 6.14±0.49mm significantly larger ([WTBX]P<0.05) than for those fed with MD, which reached at sizes ranged from 4.23±0.30mm (MD-S) to 4.46±0.30mm (MD-T). There were no significant differences in growth among MD treatments. Histological analysis of digestive epithelium of larvae showed thick and well-developed intestine folds at 12 days after hatching in both of LF and MD treatments. However, the intestine epithelium of larvae fed on MD appeared thin and most cell desquamation were observed at 18 days after hatching, indicating limited digestive capacity after 12 days after hatching and led to sharp decline of survival rate in MD treatments thereafter. Daily increments for protein, DNA and RNA as well as the ratio of RNA/DNA were higher in larvae fed LF compared to the respective values obtained for those fed MD. Larvae fed MD-T showed higher value of the above parameters than those fed MD-S and MD-U, suggesting larvae had a better nutritional condition in MD-T treatment than those in MD-S and MD-U treatments. The results indicated that supplementation of peptidase could not facilitate the digestion of MD for red sea bream larvae. However, using powder soybean lecithin and gluten could reduce the leaching rate from MD and consequently improved larval survival and growth.; 【作者单位】集美大学水产学院；东京海洋大学；日本独立行政法人水产综合研究中心养殖研究所福建厦门361021东京海洋大学；日本东京108—8477；日本三重县519—0423；【作者英文名】WANG Qiu-Rong 1, 2, Toshio Takeuchi 2 and Hirofumi Furuita 3 (1. Fisheries College, Jimei University, Ximen 361021; 2. Faculty of Marine Science, Tokyo University of Marine Science and Technology, Minato, Tokyo108—8477; 3. National Research Institut
语种	中文
内容类型	期刊论文
源URL	[http://ir.calis.edu.cn/hdl/235041/12237]
专题	集美大学
推荐引用方式 GB/T 7714	王秋荣,竹内俊郎,古板博文. 真鲷仔鱼用微粒子饲料中肽酶与大豆卵磷脂添加效果的研究(英文)[J]. http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sswx200606017&dbcode=CJFQ&dbname=CJFQ2006,2012, 2012.
APA	王秋荣,竹内俊郎,&古板博文.(2012).真鲷仔鱼用微粒子饲料中肽酶与大豆卵磷脂添加效果的研究(英文).http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sswx200606017&dbcode=CJFQ&dbname=CJFQ2006.
MLA	王秋荣,et al."真鲷仔鱼用微粒子饲料中肽酶与大豆卵磷脂添加效果的研究(英文)".http://epub.edu.cnki.net/grid2008/brief/detailj.aspx?filename=sswx200606017&dbcode=CJFQ&dbname=CJFQ2006 (2012).