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	Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif
	Xu Y(徐悦) ; Chen H(陈虎) ; Qu YJ(璩玉杰) ; Artem K.Efremov ; Li M(黎明) ; 欧阳钟灿 ; Liu DS(刘冬生) ; Yan J(严洁) ; Xu Yue ; Chen Hu ; Qu Yu-Jie ; Artem K.Efremov ; Li Ming ; Ouyang Zhong-Can ; Liu Dong-Sheng ; Yan Jie
	2016-03-30 ; 2016-03-30
关键词	single-molecule techniques i-motif folding/structure of biomolecules mechanical properties/biomolecules O561
其他题名	Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif
中文摘要	The DNA i-motif is a quadruplex structure formed in tandem cytosine-rich sequences in slightly acidic conditions.Besides being considered as a building block of DNA nano-devices, it may also play potential roles in regulating chromosome stability and gene transcriptions. The stability of i-motif is crucial for these functions. In this work, we investigated the mechanical stability of a single i-motif formed in the human telomeric sequence 5-(CCCTAA)3CCC, which revealed a novel pH and loading rate-dependent bimodal unfolding force distribution. Although the cause of the bimodal unfolding force species is not clear, we proposed a phenomenological model involving a direct unfolding favored at lower loading rate or higher pH value, which is subject to competition with another unfolding pathway through a mechanically stable intermediate state whose nature is yet to be determined. Overall, the unique mechano–chemical responses of i-motif-provide a new perspective to its stability, which may be useful to guide designing new i-motif-based DNA mechanical nano-devices.; The DNA i-motif is a quadruplex structure formed in tandem cytosine-rich sequences in slightly acidic conditions.Besides being considered as a building block of DNA nano-devices, it may also play potential roles in regulating chromosome stability and gene transcriptions. The stability of i-motif is crucial for these functions. In this work, we investigated the mechanical stability of a single i-motif formed in the human telomeric sequence 5-(CCCTAA)3CCC, which revealed a novel pH and loading rate-dependent bimodal unfolding force distribution. Although the cause of the bimodal unfolding force species is not clear, we proposed a phenomenological model involving a direct unfolding favored at lower loading rate or higher pH value, which is subject to competition with another unfolding pathway through a mechanically stable intermediate state whose nature is yet to be determined. Overall, the unique mechano–chemical responses of i-motif-provide a new perspective to its stability, which may be useful to guide designing new i-motif-based DNA mechanical nano-devices.
语种	英语 ; 英语
内容类型	期刊论文
源URL	[http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/145898]
专题	清华大学
推荐引用方式 GB/T 7714	Xu Y,Chen H,Qu YJ,et al. Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif[J],2016, 2016.
APA	徐悦.,陈虎.,璩玉杰.,Artem K.Efremov.,黎明.,...&Yan Jie.(2016).Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif..
MLA	徐悦,et al."Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif".(2016).

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