| HPLC-ELSD测定得力生注射液的黄芪甲苷的含量 | |
| 罗丹 ; 罗国安 ; 陈丽 ; 马晋芳 ; 王义明 ; LUO Dan ; LUO Guo-an ; CHEN Li ; MA Jin-fang ; WANG Yi-ming | |
| 2016-03-30 ; 2016-03-30 | |
| 关键词 | 得力生注射液 黄芪甲苷 含量测定 HPLC-ELSD municipal solid waste landfill resource utilization circular economy industrial pattern R286.0 O657 |
| 其他题名 | Determination of content of Astragaloside A in Delisheng Injection by HPLC-ELSD |
| 中文摘要 | 目的:建立得力生注射液中黄芪甲苷的含量测定方法。方法:采用高效液相色谱和蒸发光检测器的方法,Grace Alltima C18(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-水(32∶68,v/v),检测器温度100℃,载气流量为2.7L.min-1,柱温30℃,流速1 m L.min-1。进样量为20μL。结果:黄芪甲苷在1.03μg~26.2μg之间呈现良好的线性关系,r=0.9993,平均回收率为100.1%(RSD=3.7%)。结论:建立的得力生注射液中黄芪甲苷含量测定方法简单快速、准确可靠、重复性好,可有效控制得力生注射液质量。; Objective: To establish a method for the determination of Astragaloside A in Delisheng Injection. Method: The chromatographic conditions of HPLC and the parameters of ELSD were optimized to study the methodology. Analysis was performed on Grace Alltima C18 column(4.6 mm×250 mm, 5 μm) with isocratic elution of acetonitrile–water(32∶68) at the fl ow rate of 1.0 m L.min-1. Drift tube temperature was maintained at 100℃ and the gas fl ow rate was 2.7 L.min-1. Result: The calibration curve was linear in the range from 1.03 μg to 26.20 μg(r=0.9993). The recovery of astragaloside A were 100.1%(RSD=3.7%). Conclusion: This method is rapid and accurate, and can effectively control the quality of Delisheng Injection. |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://ir.lib.tsinghua.edu.cn/ir/item.do?handle=123456789/142257]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 罗丹,罗国安,陈丽,等. HPLC-ELSD测定得力生注射液的黄芪甲苷的含量[J],2016, 2016. |
| APA | 罗丹.,罗国安.,陈丽.,马晋芳.,王义明.,...&WANG Yi-ming.(2016).HPLC-ELSD测定得力生注射液的黄芪甲苷的含量.. |
| MLA | 罗丹,et al."HPLC-ELSD测定得力生注射液的黄芪甲苷的含量".(2016). |
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