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	Surface Characterization and Cytocompatibility of Three Chitosan/Polycation Composite Membranes for Guided Bone Regeneration
	Zheng, Zhenhuan ; Wei, Yujun ; Wang, Gan ; Gong, Yandao ; Zhang, Xiufang
	2010-10-12 ; 2010-10-12
关键词	chitosan surface topography AFM cytocompatibility guided bone regeneration IN-VITRO CELL INTERACTIONS CHITOSAN TISSUE SCAFFOLDS DIFFERENTIATION BIOMATERIALS ERK Engineering, Biomedical Materials Science, Biomaterials
中文摘要	Guided bone regeneration is a promising surgical procedure for reconstructing bone defects. In this study, three chitosan/polycation composite membranes for guided bone regeneration are produced by blending chitosan with poly-L-lysine, polyethyleneimine, and poly-L-ornithine. For all composite membranes, the surface characteristics including surface topography, chemistry, and wettability are examined by atomic force microscopy, X-ray photoelectron spectroscopy, and contact angle assay. Their cytocompatibility is also evaluated with MC3T3-E1 osteoblast-like cells at cell, protein, and gene levels through cell biology assays, western blot, and RT-PCR analysis. On chitosan/poly-L-lysine composite membrane, MC3T3-E1 cells present well-developed cytoskeletal organization and significantly higher adhesion, proliferation, and differentiation than those on chitosan and the other two composite membranes. Furthermore, MC3T3-E1 cells on chitosan/poly-L-lysine membrane exhibit increased phosphorylation levels of focal adhesion kinase and extracellular signal-regulated kinase 1/2, and achieve an enhanced mRNA expression of fibronectin, Runx 2, RhoA, integrin alpha 5, and integrin beta 1. From our results, we conclude that chitosan/poly-L-lysine composite membrane possesses improved cytocompatibility with osteoblasts when compared to chitosan and holds potential for guided bone regeneration in the near future.
语种	英语 ; 英语
出版者	SAGE PUBLICATIONS LTD ; LONDON ; 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81853]
专题	清华大学
推荐引用方式 GB/T 7714	Zheng, Zhenhuan,Wei, Yujun,Wang, Gan,et al. Surface Characterization and Cytocompatibility of Three Chitosan/Polycation Composite Membranes for Guided Bone Regeneration[J],2010, 2010.
APA	Zheng, Zhenhuan,Wei, Yujun,Wang, Gan,Gong, Yandao,&Zhang, Xiufang.(2010).Surface Characterization and Cytocompatibility of Three Chitosan/Polycation Composite Membranes for Guided Bone Regeneration..
MLA	Zheng, Zhenhuan,et al."Surface Characterization and Cytocompatibility of Three Chitosan/Polycation Composite Membranes for Guided Bone Regeneration".(2010).

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