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	Cloning and Characterization of Prisilkin-39, a Novel Matrix Protein Serving a Dual Role in the Prismatic Layer Formation from the Oyster Pinctada fucata
	Kong, Yawei ; Jing, Gu ; Yan, Zhenguang ; Li, Changzhong ; Gong, Ningping ; Zhu, Fangjie ; Li, Dongxian ; Zhang, Yaorun ; Zheng, Guilan ; Wang, Hongzhong ; Xie, Liping ; Zhang, Rongqing
	2010-10-12 ; 2010-10-12
关键词	JAPANESE PEARL OYSTER MOLLUSCAN SHELL PROTEINS MUSSEL PINNA-NOBILIS ORGANIC MATRIX IN-VITRO MYTILUS-CALIFORNIANUS FRAMEWORK FORMATION NACREOUS LAYER MACROMOLECULES EXPRESSION Biochemistry & Molecular Biology
中文摘要	Molluscs form their shells out of CaCO3 and a matrix of biomacromolecules. Understanding the role of matrices may shed some light on the mechanism of biomineralization. Here, a 1401-bp full-length cDNA sequence encoding a novel matrix protein was cloned from the mantle of the bivalve oyster, Pinctada fucata. The deduced protein (Prisilkin-39), which has a molecular mass of 39.3 kDa and an isoelectric point of 8.83, was fully characterized, and its role in biomineralization was demonstrated using both in vivo and in vitro crystal growth assays. Prisilkin-39 is a highly repetitive protein with an unusual composition of Gly, Tyr, and Ser residues. Expression of Prisilkin-39 was localized to columnar epithelial cells of the mantle edge, corresponding to the calcitic prismatic layer formation. Immunostaining in situ and immunodetection in vitro revealed the presence of a characteristic pattern of Prisilkin-39 in the organic sheet and in sheaths around the prisms. Prisilkin-39 binds tightly with chitin, an insoluble polysaccharide that forms the highly structured framework of the shell. Antibody injection in vivo resulted in dramatic morphological deformities in the inner shell surface structure, where large amounts of CaCO3 were deposited in an uncontrolled manner. Moreover, Prisilkin-39 strictly prohibited the precipitation of aragonite in vitro. Taken together, Prisilkin-39 is the first protein shown to have dual function, involved both in the chitinous framework building and in crystal growth regulation during the prismatic layer mineralization. These observations may extend our view on the rare group of basic matrices and their functions during elaboration of the molluscan shell.
语种	英语 ; 英语
出版者	AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC ; BETHESDA ; 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81799]
专题	清华大学
推荐引用方式 GB/T 7714	Kong, Yawei,Jing, Gu,Yan, Zhenguang,et al. Cloning and Characterization of Prisilkin-39, a Novel Matrix Protein Serving a Dual Role in the Prismatic Layer Formation from the Oyster Pinctada fucata[J],2010, 2010.
APA	Kong, Yawei.,Jing, Gu.,Yan, Zhenguang.,Li, Changzhong.,Gong, Ningping.,...&Zhang, Rongqing.(2010).Cloning and Characterization of Prisilkin-39, a Novel Matrix Protein Serving a Dual Role in the Prismatic Layer Formation from the Oyster Pinctada fucata..
MLA	Kong, Yawei,et al."Cloning and Characterization of Prisilkin-39, a Novel Matrix Protein Serving a Dual Role in the Prismatic Layer Formation from the Oyster Pinctada fucata".(2010).

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