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	Antitumor effect of F-PBF beta-TrCP-induced targeted PTTG1 degradation in HeLa cells
	Mo, Zhuohua ; Zu, Xuyu ; Xie, Zhenhua ; Li, Wenpeng ; Ning, Hang ; Jiang, Yuyang ; Xu, Wei
	2010-10-12 ; 2010-10-12
关键词	PTTG1 PTTG1-binding factor F-box Targeted degradation Growth inhibition TUMOR-TRANSFORMING GENE GREEN-FLUORESCENT PROTEIN TRANSCRIPTIONAL ACTIVATION FUNCTION UBIQUITINATION MACHINERY CHROMATID SEPARATION CELLULAR-PROTEINS FUSION PROTEINS GROWTH-FACTOR COMPLEX CANCER Biotechnology & Applied Microbiology
中文摘要	Pituitary tumor-transforming gene 1 (PTTG1), a proto-oncogene, is associated with tumor formation, proliferation and invasiveness. F-PBF beta-TrcP, a fusion protein, was produced by replacing the WD40-repeat of F-box protein beta-TrCP with the PTTG1-binding factor (PBF) for targeted degradation of PTTG1. To evaluate the function of F-PBF beta-TrcP, PTTG1-EGFP fusion protein was constructed. Our results showed that F-PBF beta-TrcP can both degrade exogenous PTTG1-EGFP fusion protein in COS-7 cells and endogenous PTTG1 protein in HeLa cells and the targeted PTTG1 knock down resulted in bFGF mRNA level down-regulation and inhibition of proliferation and clonogenicity in HeLa cells. In conclusion, targeted degradation of PTTG1 by F-PBF beta-TrcP has antitumor activity in vitro in HeLa cells. These results suggest that F-PBF beta-TrcP could be used for cancer treatment by targeted degradation of PTTG1. (c) 2008 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81701]
专题	清华大学
推荐引用方式 GB/T 7714	Mo, Zhuohua,Zu, Xuyu,Xie, Zhenhua,et al. Antitumor effect of F-PBF beta-TrCP-induced targeted PTTG1 degradation in HeLa cells[J],2010, 2010.
APA	Mo, Zhuohua.,Zu, Xuyu.,Xie, Zhenhua.,Li, Wenpeng.,Ning, Hang.,...&Xu, Wei.(2010).Antitumor effect of F-PBF beta-TrCP-induced targeted PTTG1 degradation in HeLa cells..
MLA	Mo, Zhuohua,et al."Antitumor effect of F-PBF beta-TrCP-induced targeted PTTG1 degradation in HeLa cells".(2010).

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