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	Study of the Relationship between Apoptosis and Intracellular pH in Single Living Cells Using a Three-Channel Real-Time Fluorescence Imaging Method
	Lin Dan-ying ; Liu Xiao-chen ; Ma Wan-yun
	2010-10-12 ; 2010-10-12
关键词	Three-channel Real-time Fluorescence Imaging Living cells Apoptosis Intracellular pH INDUCTION EMBRYO RAT Spectroscopy
中文摘要	Using a Dual-View wavelength splitter, a custom-made filter block and only a single intensified charge-coupled device (ICCD), a three-channel real-time fluorescence imaging method for single living cell research was established based on an ICCD fast fluorescence micro-imaging system. The relevant calibrating method for images was also developed to eliminate the influence introduced by spectral crosstalk. Double-labeled with two fluorescent indicators, Annexin V-FITC and SNARF-1, mouse thymocyte was treated with S-nitrosoglutathione (GSNO) and the relationship between their apoptosis and intracellular pH changes was studied in real-time at the single living cell level. The results not only showed the different regularities of intracellular pH changes between apoptosis induced by GSNO and spontaneous apoptosis, but also provided evidence for the application of the three-channel real-time fluorescence imaging method to bio-medical studies.
语种	中文 ; 中文
出版者	OFFICE SPECTROSCOPY & SPECTRAL ANALYSIS ; BEIJING ; NO 76 COLLAGE SOUTH RD BEIJING, BEIJING 100081, PEOPLES R CHINA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81519]
专题	清华大学
推荐引用方式 GB/T 7714	Lin Dan-ying,Liu Xiao-chen,Ma Wan-yun. Study of the Relationship between Apoptosis and Intracellular pH in Single Living Cells Using a Three-Channel Real-Time Fluorescence Imaging Method[J],2010, 2010.
APA	Lin Dan-ying,Liu Xiao-chen,&Ma Wan-yun.(2010).Study of the Relationship between Apoptosis and Intracellular pH in Single Living Cells Using a Three-Channel Real-Time Fluorescence Imaging Method..
MLA	Lin Dan-ying,et al."Study of the Relationship between Apoptosis and Intracellular pH in Single Living Cells Using a Three-Channel Real-Time Fluorescence Imaging Method".(2010).

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