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	Enantiomeric Separation of Two Antiparkinsonian Drugs by Electrokinetic Chromatography Using Dextran Sulfate
	Chu, Bao-Lin ; Feng, Qinzhong ; Wang, Zhihua ; Lin, Jin-Ming
	2010-10-12 ; 2010-10-12
关键词	Capillary electrophoresis Chiral separation Dextran sulfate Rotigotine Trihexyphenidyl CAPILLARY-ZONE-ELECTROPHORESIS CHIRAL SELECTORS LIQUID-CHROMATOGRAPHY BETA-CYCLODEXTRIN HUMAN SERUM ENANTIOSEPARATION POLYSACCHARIDES TRIHEXYPHENIDYL ROTIGOTINE ADDITIVES Biochemical Research Methods Chemistry, Analytical
中文摘要	Anionic polysaccharide dextran sulfate (DxS) was successfully employed as chiral selector for the enantioseparation of two antiparkinsonian drugs, including rotigotine and trihexyphenidyl (THP), by electrokinetic chromatography (EKC). The enantioseparation was performed under normal and reversed polarity modes and reversed enantiomer migration order was achieved under two modes. The parameters affecting the chiral separation, such as buffer pH, DxS concentration, organic additive, and temperature were investigated and optimized. Reversed polarity mode provided better separation for the two drugs. The optimized conditions for the enantioseparation under reversed polarity mode were 2.0% (w/v) DxS, 10 mM phosphate buffer, pH 2.5 with an applied voltage of -30 kV at 25 A degrees C. Direct UV detection was performed at 200 nm. Under the optimal conditions, rotigotine and THP enantiomers were enantioresolved in 40 min with the resolution of 2.0 and 5.8, respectively. The analytes could be enantioseparated using DxS of molecular mass 1,000,000 or 500,000. It was inferred that the electrostatic, hydrophobic, and steric interactions may be involved in the chiral separation mechanism in this study.
语种	英语 ; 英语
出版者	VIEWEG ; WIESBADEN ; ABRAHAM-LINCOLN-STRABE 46, POSTFACH 15 47, D-65005 WIESBADEN, GERMANY
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81148]
专题	清华大学
推荐引用方式 GB/T 7714	Chu, Bao-Lin,Feng, Qinzhong,Wang, Zhihua,et al. Enantiomeric Separation of Two Antiparkinsonian Drugs by Electrokinetic Chromatography Using Dextran Sulfate[J],2010, 2010.
APA	Chu, Bao-Lin,Feng, Qinzhong,Wang, Zhihua,&Lin, Jin-Ming.(2010).Enantiomeric Separation of Two Antiparkinsonian Drugs by Electrokinetic Chromatography Using Dextran Sulfate..
MLA	Chu, Bao-Lin,et al."Enantiomeric Separation of Two Antiparkinsonian Drugs by Electrokinetic Chromatography Using Dextran Sulfate".(2010).

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