QQ客服 官方微博 反馈留言

	A New Strategy for Highly Sensitive Immunoassay Based on Single-Particle Mode Detection by Inductively Coupled Plasma Mass Spectrometry
	Hu, Shenghong ; Liu, Rui ; Zhang, Sichun ; Huang, Zhi ; Xing, Zhi ; Zhang, Xinrong
	2010-10-12 ; 2010-10-12
关键词	ELEMENT-TAGGED IMMUNOASSAY TIME-RESOLVED FLUOROIMMUNOASSAY COLLOIDAL GOLD LABEL ALPHA-FETOPROTEIN QUANTUM DOTS HUMAN SERUM ICP-MS NANOPARTICLES RESONANCE PROTEINS Chemistry, Analytical Chemistry, Physical Spectroscopy
中文摘要	A highly sensitive immunoassay is proposed based on time-resolved inductively coupled plasma mass spectrometry with nanoparticles as tags to antibody. Instead of using traditional integral mode detection, the transient signals induced by the flash of ions in the plasma torch from the ionization of nanoparticles tagged on antibody were recorded in a time-resolved mode. Since, under certain conditions, the frequency of transient signals is directly correlated to the concentration of nanoparticle tags, the concentration of nanoparticle-tagged antibodies can be quantified by the frequency of transient signals. With the present instrument setup, gold nanoparticle (Au-NP) tags, as small as about 15 nm in diameter, can be detected. This protocol is evaluated for a competitive immunoassay and the linear range for a-fetoprotein is 0.016-6.8 mu g/L (between 20 and 80% inhibition). The limit of quantification is 0.016 mu g/L (20% inhibition, IC20) with a relative standard deviation of 4.2% (20% inhibition, 4 replicates) for a-fetoprotein. The present strategy provides a sensitive readout method for nanoparticle tags, which is quite promising for numerous applications in immunoassay, DNA hybridization, and other biological analyses. (J Am Soc Mass Spectrom 2009, 20, 1096-1103) (C) 2009 American Society for Mass Spectrometry
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE INC ; NEW YORK ; 360 PARK AVE SOUTH, NEW YORK, NY 10010-1710 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/81146]
专题	清华大学
推荐引用方式 GB/T 7714	Hu, Shenghong,Liu, Rui,Zhang, Sichun,et al. A New Strategy for Highly Sensitive Immunoassay Based on Single-Particle Mode Detection by Inductively Coupled Plasma Mass Spectrometry[J],2010, 2010.
APA	Hu, Shenghong,Liu, Rui,Zhang, Sichun,Huang, Zhi,Xing, Zhi,&Zhang, Xinrong.(2010).A New Strategy for Highly Sensitive Immunoassay Based on Single-Particle Mode Detection by Inductively Coupled Plasma Mass Spectrometry..
MLA	Hu, Shenghong,et al."A New Strategy for Highly Sensitive Immunoassay Based on Single-Particle Mode Detection by Inductively Coupled Plasma Mass Spectrometry".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。