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	Controlled Adipose-derived Stromal Cells Differentiation into Adipose and Endothelial Cells in a 3D Structure Established by Cell-assembly Technique
	Xu, Mingen ; Yan, Yongnian ; Liu, Haixia ; Yao, Rui ; Wang, Xiaohong
	2010-10-12 ; 2010-10-12
关键词	adipose-derived stromal (ADS) cells cell-assembly technique vascularize endothelial vessel network adipose-tissue engineering tissue engineering TISSUE-ENGINEERED CONSTRUCTS IN-VITRO BIOARTIFICIAL LIVERS POLYURETHANE ROSIGLITAZONE REGENERATION ADIPOKINES SCAFFOLDS CREATION LIPIDS Biotechnology & Applied Microbiology Materials Science, Biomaterials Polymer Science
中文摘要	One of the major obstacles in engineering thick and complex tissues while vascularizing tissues in vitro is to maintain cell viability during tissue growth and structural organization. Adipose-derived stromal (ADS) cells were used to establish a multicellular system through a cell-assembly technique. Attempts were made to control ADS cells differentiation into different targeted cell types according to their positions within an orderly 3D structure. Oil red O staining confirmed that the ADS cells in the structure differentiated into adipocytes with a spherical shape while immunostaining tests confirmed that the endothelial growth factor induced ADS cells on the walls of channels differentiated into mature endothelial cells and then organized into tubular structures throughout the engineered 3D structure. The endothelin-1 and nitric oxide release rules of the endothelial cells were coincidental with those in vivo. This study provides a new approach to engineer orderly endothelial vessel networks in vitro and has potential applications in adipose-tissue engineering.
语种	英语 ; 英语
出版者	SAGE PUBLICATIONS LTD ; LONDON ; 1 OLIVERS YARD, 55 CITY ROAD, LONDON EC1Y 1SP, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/80479]
专题	清华大学
推荐引用方式 GB/T 7714	Xu, Mingen,Yan, Yongnian,Liu, Haixia,et al. Controlled Adipose-derived Stromal Cells Differentiation into Adipose and Endothelial Cells in a 3D Structure Established by Cell-assembly Technique[J],2010, 2010.
APA	Xu, Mingen,Yan, Yongnian,Liu, Haixia,Yao, Rui,&Wang, Xiaohong.(2010).Controlled Adipose-derived Stromal Cells Differentiation into Adipose and Endothelial Cells in a 3D Structure Established by Cell-assembly Technique..
MLA	Xu, Mingen,et al."Controlled Adipose-derived Stromal Cells Differentiation into Adipose and Endothelial Cells in a 3D Structure Established by Cell-assembly Technique".(2010).

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