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	Structural Basis of Novel Interactions Between the Small-GTPase and GDI-like Domains in Prokaryotic FeoB Iron Transporter
	Hattori, Motoyuki ; Jin, Yaohua ; Nishimasu, Hiroshi ; Tanaka, Yoshiki ; Mochizuki, Masahiro ; Uchiumi, Toshio ; Ishitani, Ryuichiro ; Ito, Koichi ; Nureki, Osamu
	2010-10-12 ; 2010-10-12
关键词	GUANINE-NUCLEOTIDE-BINDING TRANSFER-RNA MODIFICATION RAY DIFFRACTION ANALYSIS MEMBRANE-PROTEIN FEOB ESCHERICHIA-COLI CRYSTAL-STRUCTURE CYTOSOLIC DOMAIN FERROUS IRON CRYSTALLIZATION SOFTWARE Biochemistry & Molecular Biology Biophysics Cell Biology
中文摘要	The FeoB family proteins are widely distributed prokaryotic membrane proteins involved in Fe2+ uptake. FeoB consists of N-terminal cytosolic and C-terminal transmembrane domains. The N-terminal region of the cytosolic domain is homologous to small GTPase (G) proteins and is considered to regulate Fe2+ uptake. The spacer region connecting the G and TM domains reportedly functions as a GDP dissociation inhibitor (GDI)-like domain that stabilizes the GDP-binding state. However, the function of the G and GDI-like domains in iron uptake remains unclear. Here, we report the structural and functional analyses of the FeoB cytosolic domain from Thermotoga maritima. The structure-based mutational analysis indicated that the interaction between the G and GDI-like domains is important for both the GDI and Fe2+ uptake activities. On the basis of these results, we propose a regulatory mechanism of Fe2+ uptake.
语种	英语 ; 英语
出版者	CELL PRESS ; CAMBRIDGE ; 600 TECHNOLOGY SQUARE, 5TH FLOOR, CAMBRIDGE, MA 02139 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/79567]
专题	清华大学
推荐引用方式 GB/T 7714	Hattori, Motoyuki,Jin, Yaohua,Nishimasu, Hiroshi,et al. Structural Basis of Novel Interactions Between the Small-GTPase and GDI-like Domains in Prokaryotic FeoB Iron Transporter[J],2010, 2010.
APA	Hattori, Motoyuki.,Jin, Yaohua.,Nishimasu, Hiroshi.,Tanaka, Yoshiki.,Mochizuki, Masahiro.,...&Nureki, Osamu.(2010).Structural Basis of Novel Interactions Between the Small-GTPase and GDI-like Domains in Prokaryotic FeoB Iron Transporter..
MLA	Hattori, Motoyuki,et al."Structural Basis of Novel Interactions Between the Small-GTPase and GDI-like Domains in Prokaryotic FeoB Iron Transporter".(2010).

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