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	Metabolism in 1,3-Propanediol Fed-Batch Fermentation by a D-Lactate Deficient Mutant of Klebsiella pneumoniae
	Xu, Yun-Zhen ; Guo, Ni-Ni ; Zheng, Zong-Ming ; Ou, Xian-Jin ; Liu, Hong-Juan ; Liu, De-Hua
	2010-10-12 ; 2010-10-12
关键词	1,3-Propanediol D-lactate dehydrogenase fed-batch internal redox state ANAEROBIC CONTINUOUS-CULTURE ESCHERICHIA-COLI-B CLOSTRIDIUM-BUTYRICUM GLYCEROL FERMENTATION DIOL-DEHYDRATASE DEHYDROGENASE PATHWAY 2,3-BUTANEDIOL ACID GENE Biotechnology & Applied Microbiology
中文摘要	Klebsiella pneumoniae HR526, a new isolated 1,3-Propanediol (1,3-PD) producer, exhibited great productivity. However, the accumulation of lactate in the late-exponential phase remained an obstacle of 1,3-PD industrial scale production. Hereby, mutants lacking D-lactate pathway were constructed by knocking out the ldhA gene encoding fermentative D-lactate dehydrogenase (LDH) of HR526. The mutant K. pneumoniae LDH526 with the lowest LDH activity was studied in aerobic fed-batch fermentation. In experiments, conversion; and productivity increased from 95.39 gL(-1), 0.48 and 1.98 gL(-1)h(-1) to 102.06 gL(-1,) 0.52 mol mol(-1) and 2.13 gL(-1)h(-1), respectively. The diol (1,3-PD and 2,3-butanediol) conversion increased from 0.55 mol mol(-1) to a maximum of 0.65 mol mol(-1). Lactate would not accumulate until 1,3-PD exceeded 84 gL(-1), and the final lactate concentration decreased dramatically from more than 40 gL(-1) to <3 gL(-1). Enzymic measurements showed LDH activity decreased by 89-98% during fed-batch fermentation, and other related enzyme activities were not affected. NADH/NAD(+) enhanced more than 50% in the late-exponential phase as the D-lactate pathway was cut off, which might be the main reason for the change of final metabolites concentrations. The ability to utilize crude glycerol from biodiesel process and great genetic stability demonstrated that K. pnemoniae LDH526 was valuable for 1,3-PD industrial production. Biotechnol Bioeng. 2009;104: 965-972. (C) Wiley Periodicals, Inc.
语种	英语 ; 英语
出版者	JOHN WILEY & SONS INC ; HOBOKEN ; 111 RIVER ST, HOBOKEN, NJ 07030 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/79523]
专题	清华大学
推荐引用方式 GB/T 7714	Xu, Yun-Zhen,Guo, Ni-Ni,Zheng, Zong-Ming,et al. Metabolism in 1,3-Propanediol Fed-Batch Fermentation by a D-Lactate Deficient Mutant of Klebsiella pneumoniae[J],2010, 2010.
APA	Xu, Yun-Zhen,Guo, Ni-Ni,Zheng, Zong-Ming,Ou, Xian-Jin,Liu, Hong-Juan,&Liu, De-Hua.(2010).Metabolism in 1,3-Propanediol Fed-Batch Fermentation by a D-Lactate Deficient Mutant of Klebsiella pneumoniae..
MLA	Xu, Yun-Zhen,et al."Metabolism in 1,3-Propanediol Fed-Batch Fermentation by a D-Lactate Deficient Mutant of Klebsiella pneumoniae".(2010).

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