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	Purification, crystallization and preliminary X-ray analysis of cytochrome P450 219A1 from Novosphingobium aromaticivorans DSM 12444
	Hong, Chuan ; Bell, Stephen G. ; Yang, Wen ; Wang, Hui ; Hao, Yiming ; Li, Xin ; Zhou, Weihong ; Bartlam, Mark ; Wong, Luet-Lok
	2010-10-12 ; 2010-10-12
关键词	P450 ENZYMES COMPLEX SYSTEM Biochemical Research Methods Biochemistry & Molecular Biology Biophysics Crystallography
中文摘要	Cytochrome P450 enzymes catalyze a variety of reactions and are widely distributed in living organisms. In recent studies, the first members of five new families of cytochrome P450 enzymes have been identified, including cytochrome P450 219A1 (CYP219A1) from Novosphingobium aromaticivorans DSM 12444. It has also been reported that isolongifolen-9-one (C15H22O), a sesquiterpenoid ketone derivative, is a potential substrate for CYP219A1, inducing a >= 95% shift of the haem spin state to high spin upon binding. The CYP219A1 protein has been crystallized and single crystals have been studied by X-ray crystallography. Diffraction data were collected to 2.4 angstrom resolution. The crystals belonged to space group P6, with unit-cell parameters a = 93.1, b = 93.1, c = 98.0 angstrom. Preliminary X-ray diffraction data analysis revealed that the asymmetric unit contained one protein molecule.
语种	英语 ; 英语
出版者	WILEY-BLACKWELL PUBLISHING, INC ; MALDEN ; COMMERCE PLACE, 350 MAIN ST, MALDEN 02148, MA USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/78686]
专题	清华大学
推荐引用方式 GB/T 7714	Hong, Chuan,Bell, Stephen G.,Yang, Wen,et al. Purification, crystallization and preliminary X-ray analysis of cytochrome P450 219A1 from Novosphingobium aromaticivorans DSM 12444[J],2010, 2010.
APA	Hong, Chuan.,Bell, Stephen G..,Yang, Wen.,Wang, Hui.,Hao, Yiming.,...&Wong, Luet-Lok.(2010).Purification, crystallization and preliminary X-ray analysis of cytochrome P450 219A1 from Novosphingobium aromaticivorans DSM 12444..
MLA	Hong, Chuan,et al."Purification, crystallization and preliminary X-ray analysis of cytochrome P450 219A1 from Novosphingobium aromaticivorans DSM 12444".(2010).

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