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	Preparation and characterization of chitosan microspheres for controlled release of synthetic oligopeptide derived from BMP-2
	Niu, Xufeng ; Feng, Qingling ; Wang, Mingbo ; Guo, Xiaodong ; Zheng, Qixin
	2010-10-12 ; 2010-10-12
关键词	Chitosan microspheres bone morphogenetic protein oligopeptide controlled release BIODEGRADABLE POLYMER MICROPARTICLES BONE MORPHOGENETIC PROTEIN-2 GROWTH-FACTOR BETA-1 VITAMIN-C DRUG-DELIVERY SCAFFOLDS CARRIERS EMULSIFICATION REGENERATION OSTEOGENESIS Chemistry, Applied Engineering, Chemical Pharmacology & Pharmacy
中文摘要	The localized and temporally controlled release of growth factors is key to achieving optimal clinical efficacy. To achieve sustained delivery of a novel bone-induced growth factor, chitosan microspheres loaded with synthetic oligopeptide (S-[PO4] KIPKASSVPTELSAISTLYLDDD, P24) were prepared by an emulsion-ionic cross-linking method in the presence of tripolyphosphate, with bovine serum albumin (BSA) as a control. Both microspheres containing oligopeptide or BSA were of spherical shape with size ranging from 10-60 mu m. The encapsulation efficiency was usually higher than 80% and the loading capacity was affected by initial protein dosage. From the release experiments, it was found that both proteins were slowly released from the microspheres over 7 days in a PBS solution (pH 7.4), in which the release rate of oligopeptide was much lower than that of BSA. Released oligopeptide was demonstrated to possess biological activity as evidenced by stimulation of rabbit marrow mesenchymal stem cells (MSCs) alkaline phosphatase (ALP) activity in vitro. These results indicate that the TPP-chitosan microspheres loaded with synthetic oligopeptide may possess potential application in bone tissue engineering.
语种	英语 ; 英语
出版者	TAYLOR & FRANCIS LTD ; ABINGDON ; 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/78332]
专题	清华大学
推荐引用方式 GB/T 7714	Niu, Xufeng,Feng, Qingling,Wang, Mingbo,et al. Preparation and characterization of chitosan microspheres for controlled release of synthetic oligopeptide derived from BMP-2[J],2010, 2010.
APA	Niu, Xufeng,Feng, Qingling,Wang, Mingbo,Guo, Xiaodong,&Zheng, Qixin.(2010).Preparation and characterization of chitosan microspheres for controlled release of synthetic oligopeptide derived from BMP-2..
MLA	Niu, Xufeng,et al."Preparation and characterization of chitosan microspheres for controlled release of synthetic oligopeptide derived from BMP-2".(2010).

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