QQ客服 官方微博 反馈留言

	内植物感染生物被膜动物模型的建立与评价
	储诚兵 ; 黎檀实 ; 沈定霞 ; 王慧 ; 曹敬荣 ; 王继芳 ; CHU Cheng-bing ; LI Tan-shi ; SHEN Ding-xia ; WANG Hui ; CAO Jing-rong ; WANF Ji-fang
	2010-06-10 ; 2010-06-10
关键词	内植物 感染 生物被膜 疾病模型 动物 Implant Infection Biofilm Disease model Animal R-332
其他题名	Animal Model with Implant Infection by Biofilm:Establishment and Evaluation
中文摘要	目的旨在建立兔内植物感染生物被膜模型,为研究生物被膜提供基础。方法对表皮葡萄球菌(SEP)培养、纯化、鉴定、药敏及生物被膜形成实验;健康成年新西兰大白兔随机分为4组,每组8只;麻醉满意后,在兔右后膝关节内股骨外髁非关节面处置入不锈钢螺钉及超高分子量聚乙烯(UHMWPE)垫圈,缝合伤口,4组兔膝关节腔分别接种1 ml无菌生理盐水,102、103、104CFU SEP;术后观察伤口情况,第14天处死大白兔,无菌取出兔膝关节滑膜组织行细菌培养,确定膝关节有无感染及SEP感染膝关节的适宜剂量;扫描电镜(SEM)、激光共聚焦显微镜(LCSM)观察适宜感染剂量组UHMWPE垫片表面生物被膜、SEP及细胞外黏多糖分布情况。结果菌株鉴定为SEP、可形成生物被膜;生理盐水组、102、103、104CFU SEP组,兔膝关节感染率分别为0、37.5%、100.0%、100.0%,伤口愈合不良4组分别为0、1、2、4只,表明103CFU SEP为适宜的膝关节感染剂量(ID);SEM及LCSM观察103CFU SEP组UHMWPE垫片表面均形成生物被膜;SEM观察UHMWPE垫片表面生物被膜内SEP聚集,SEP被基质包裹;LCSM观察UHMWPE垫片表面生物被膜结构为红色荧光的SEP镶嵌在绿色荧光的黏多糖内。结论此模型可为研究生物被膜提供一种有效的方法。; OBJECTIVE To establish the implanted biofilm model of rabbit implant infection.METHODS SEP was cultured,purified,identified,and tested for the drug sensitivity and the biofilm production.Healthy adult rabbits were randomly divided into 4 groups,8 in each group.The stainless steel screws and washer UHMWPEs were implanted into the femoral condyle of the non-articular surface of the rabbit right stifle knee.The knee joints were inoculated with 1ml sterile saline,102,103 and 104 CFU SEP,respectively.Wounds were observed postoperatively.After the 14th days postoperation,the knee synovium of 32 rabbits sacrificed were taken out by aseptic technique and were cultured to determine whether the knees were infected and which concentration of SEP was the appropriate for causing knee joint infection.UHMWPEs from the appropriate ID group were observed to find whether there were biofilms on the surface by SEM and LCSM.RESULTS The bacterial strain was identified as SEP and could produce biofilm.Among the knee joints inoculated with 1ml saline,102,103 and 104 CFU SEP,the infective rate was 0,37.5%,100.0% and 100.0% and poor wound healing was in 0,1,2 and 4 rabbits,respectively.It showed 103 CFU of SEP was the appropriate ID.Biofilms were found on all UHMWPE surfaces from 103 CFU SEP group by SEM and LCSM.SEM showed SEP in the biofilms on the surface of UHMWPE was agglomerated and wrapped in the matrix.The structure of biofilms in which SEP radiated red fluorescence was inlaid in mucopolysaccharide stained green fluorescence was observed by LCSM.CONCLUSIONS The model provides an effective method to investigate the biofilm.
语种	中文 ; 中文
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/61506]
专题	清华大学
推荐引用方式 GB/T 7714	储诚兵,黎檀实,沈定霞,等. 内植物感染生物被膜动物模型的建立与评价[J],2010, 2010.
APA	储诚兵.,黎檀实.,沈定霞.,王慧.,曹敬荣.,...&WANF Ji-fang.(2010).内植物感染生物被膜动物模型的建立与评价..
MLA	储诚兵,et al."内植物感染生物被膜动物模型的建立与评价".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。