| 荧光探针法研究色氨酸二肽与DNA的相互作用 | |
| 卢奎 ; 冯业振 ; 赵东欣 ; 任海平 ; 赵玉芬 ; LU Kui ; FENG Ye-zhen ; ZHAO Dong-xin ; REN Hai-ping ; ZHAO Yu-fen | |
| 2010-06-10 ; 2010-06-10 | |
| 关键词 | 荧光光谱 色氨酸二肽 小牛胸腺DNA 相互作用 Fluorescence spectrum Tryptophan dipeptide Calf Thymus DNA Interaction O629.7 |
| 其他题名 | Study on the interaction of trp-trp and DNA by fluorescence probe |
| 中文摘要 | 以溴化乙锭(EB)为荧光探针,对色氨酸二肽(Trp-Trp)与小牛胸腺DNA的作用机制进行了研究,考查了酸度、离子强度及磷酸根离子对Trp-Trp与DNA相互作用的影响;结果表明,在pH 7.2的模拟体液条件下,Trp-Trp可与DNA发生相互作用;向DNA-EB体系中加入Trp-Trp时荧光逐渐减弱,这说明Trp-Trp能与DNA发生作用。通过Scatchard方程进一步分析发现,Trp-Trp对DNA-EB的影响表现为混合模式:一方面,Trp-Trp能中和DNA上的磷氧负离子,导致DNA链收缩,从而影响DNA的构象,使嵌入的EB从DNA中部分游离出来,荧光减弱;另一方面,Trp-Trp与EB也存在静电位点竞争。; The interaction between tryptophan dipeptide (Trp-Trp) and ct-DNA was studied by fluorescence spectros- copy using ethidium bromide (EB) as fluorescence probe,the effect factors such as acidity,salt effect and phosphoric acid ion on the interaction are investigated;It is showed that in Tris-HCl (pH 7.2),Trp-Trp can react with DNA. The fluorescence intensity of DNA-EB quenched with the increase of Trp-Trp.According to the equation of scatchard, influence of Trp-Trp on interaction of DNA-EB belongs to mixed model.On the one hand,the oxyphosphate anion of DNA can be neutralized by Trp-Trp partially,which induced the contraction of DNA and the conformational change of DNA,leading to release of EB from DNA partially;on the other hand,the Trp-Trp can also compete the electrostatic sites with EB directly.; 国家自然科学基金(20272055，20572016); 河南省杰出青年基金(0312000900)项目资助 |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/61017]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 卢奎,冯业振,赵东欣,等. 荧光探针法研究色氨酸二肽与DNA的相互作用[J],2010, 2010. |
| APA | 卢奎.,冯业振.,赵东欣.,任海平.,赵玉芬.,...&ZHAO Yu-fen.(2010).荧光探针法研究色氨酸二肽与DNA的相互作用.. |
| MLA | 卢奎,et al."荧光探针法研究色氨酸二肽与DNA的相互作用".(2010). |
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