| 纳米转基因技术可用于大鼠心梗后血管再生 | |
| 唐红梅 ; 廉瑞青 ; 张晓东 ; TANG Hong-mei ; LIAN Rui-qing ; ZHANG Xiao-dong | |
| 2010-05-13 ; 2010-05-13 | |
| 关键词 | 心肌梗塞 纳米粒子 血管再生 转基因技术 myocardial infarction nanoparticle angiogenesis transgenic technology R542.22 |
| 其他题名 | Nanometer transgenic technology and angiogenesis after myocardial infarction in rats |
| 中文摘要 | 目的探讨包载VEGF165质粒的聚乳酸-乙醇酸(PLGA)纳米粒子对大鼠心肌梗死后血管再生治疗的可行性。方法制备大鼠心梗模型36只,实验组24只、对照组12只,术后1周在梗死区和与正常心肌交界处注射pVEGF165-PLGA纳米粒子和pVEGF165。应用RT-PCR和免疫组化检测血管内皮生长因子在不同的时间点(3、7、11、14和21 d)的表达;组织切片观察梗死区血管形成特点及其密度;以及纳米粒子对人体的毒副作用。结果与对照组相比,实验组VEGF可在心肌组织持续稳定表达;梗死区血管内皮细胞增生活跃,再生血管数量增加。注射48 h后心肌细胞核内可见纳米粒子,8周后组织活检未见血管瘤。结论PLGA纳米粒子有效介导pVEGF165转染心肌,并通过心肌细胞表达VEGF165,促进缺血区心肌组织的血管再生。; Objective To investigate the feasibility of the gene therapy on angiogenesis after myocardial infarction in rats.Methods Thirty-six male SD rats,after the ligation of left anterior descending coronary artery,were divided into 2 groups as experimental and control one.Expressions of VEGF were measured by RT-PCR and Immunohistochemistry(IHC).Angiogenesis and capillary density were evaluated by HE stain,and qualitative and quantitative analysis were carried out.The adverse effects were tested after injection of pVEGF165-PLGA nanoparticle.Results Compared with control groups,ischemic myocardial cells persistently and stably expressed VEGF in experimental group;Vascular endothelial cells actively proliferated,and the effect of angiogenesis was significant;48 hours later,nanoparticles were observed in myocardial cells.Conclusion Injection of with pVEGF165-PLGA nanoparticle,it can stimulate effective host-derived angiogenesis,which results in the prevention of impaired cardiac muscle after myocardial infarction.It may be an effect way to treat MI. |
| 语种 | 中文 ; 中文 |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/32649]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | 唐红梅,廉瑞青,张晓东,等. 纳米转基因技术可用于大鼠心梗后血管再生[J],2010, 2010. |
| APA | 唐红梅,廉瑞青,张晓东,TANG Hong-mei,LIAN Rui-qing,&ZHANG Xiao-dong.(2010).纳米转基因技术可用于大鼠心梗后血管再生.. |
| MLA | 唐红梅,et al."纳米转基因技术可用于大鼠心梗后血管再生".(2010). |
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